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Team:Trieste/parts/3
From 2012.igem.org
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</br> | </br> | ||
<h2>Results</h2> | <h2>Results</h2> | ||
| + | CymR expression was confirmed by Western Blot analysis. | ||
| + | Our CymR has a SV5 tag at the C-terminus in order to be detected by an anti-SV5 Ab. | ||
| + | Its functionality was confirmed through the repression of T5 Cumate Operator-GFP. | ||
| + | |||
| + | <img src="https://static.igem.org/mediawiki/2012/d/d0/Trieste-CymR_V.jpg" width="500px"/> | ||
| + | |||
</br> | </br> | ||
<h2>Modelling</h2> | <h2>Modelling</h2> | ||
Revision as of 14:15, 25 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
BBa_K875003
More
Description
This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter.Assembly
Results
CymR expression was confirmed by Western Blot analysis. Our CymR has a SV5 tag at the C-terminus in order to be detected by an anti-SV5 Ab. Its functionality was confirmed through the repression of T5 Cumate Operator-GFP.
Modelling
Looking forward
Link to the Registry
