Team:Exeter/lab book/proto/2

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Template:Team:Exeter/e-candi_banner}} <html xmlns="http://www.w3.org/1999/xhtml"> <head> <meta http-equiv="Content-Type" content="text/html; charset=utf-8" /> <title>Protocol ...")
m (added last line of text)
Line 61: Line 61:
     <li><p>Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.</p></li>
     <li><p>Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.</p></li>
     <li><p>Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.</p></li>
     <li><p>Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.</p></li>
 +
    <li><p>Incubate cultures overnight in a shaking incubator ~225 rpm, 37 °C.</p></li>
     </ul>   
     </ul>   
   </font>
   </font>

Revision as of 12:07, 25 September 2012

Protocol 2

Transferring Cultures to Liquid Medium

  • Defrost antibiotic for selection, corresponding to the plate antibiotic. Add antibiotic to 5ml of LB broth corresponding to a 1000 fold dilution.

  • Select three colonies from each plate and inoculate to 5ml of LB media via a pipette tip.

  • Incubate cultures overnight in a shaking incubator ~225 rpm, 37 °C.

Retrieved from "http://2012.igem.org/Team:Exeter/lab_book/proto/2"