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Team:LMU-Munich/Data/MazF
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== MazF cloning in ''E. coli''== | == MazF cloning in ''E. coli''== | ||
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<p align="justify"> The cloning of MazF in ''E. coli'' itself was tricky. Even though the MazF itself worked, it didn't grow well, and after retransformation we got only six colonies to pick (right picture above). This shows that this Biobrick is probably functional and is a problem for ''E. coli'' to handle even without promoter.<br></p> | <p align="justify"> The cloning of MazF in ''E. coli'' itself was tricky. Even though the MazF itself worked, it didn't grow well, and after retransformation we got only six colonies to pick (right picture above). This shows that this Biobrick is probably functional and is a problem for ''E. coli'' to handle even without promoter.<br></p> | ||
Revision as of 11:18, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
MazF cloning in E. coli
The cloning of MazF in E. coli itself was tricky. Even though the MazF itself worked, it didn't grow well, and after retransformation we got only six colonies to pick (right picture above). This shows that this Biobrick is probably functional and is a problem for E. coli to handle even without promoter.
The 3a assembly with the promoter did not work at all (left picture above). We yielded mostly just red colonies and the few that were white were wrong, never having the promoter with MazF. After many attempts with either the pSB1C3 or our own Bacillus vectors, we chose to directly transform into Bacillus subtilis in hope that it would cope better with the toxin and/or the promoter would be less leaky. This was though sadly too late to finish before Amsterdam. But maybe the story will be told to an end at Boston.
