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Team:LMU-Munich/Bacillus BioBricks/integration
From 2012.igem.org
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|[[File:LMU-Munich-integration-Picture1.png|500px|center]] | |[[File:LMU-Munich-integration-Picture1.png|500px|center]] | ||
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| + | |'''A''': ''Bacillus'' vector | ||
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|[[File:LMU-Munich-integration-Arrow.png|80px|center]] | |[[File:LMU-Munich-integration-Arrow.png|80px|center]] | ||
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|[[File:LMU-Munich-integration-Picture2.png|500px|center]] | |[[File:LMU-Munich-integration-Picture2.png|500px|center]] | ||
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| + | |'''B''': Linearisation with ''Sca''I | ||
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|[[File:LMU-Munich-integration-Arrow.png|80px|center]] | |[[File:LMU-Munich-integration-Arrow.png|80px|center]] | ||
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|[[File:LMU-Munich-integration-Picture3.png|500px|center]] | |[[File:LMU-Munich-integration-Picture3.png|500px|center]] | ||
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| + | |'''C''': Linearized vector | ||
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|[[File:LMU-Munich-integration-Arrow.png|80px|center]] | |[[File:LMU-Munich-integration-Arrow.png|80px|center]] | ||
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|[[File:LMU-Munich-integration-Picture4.png|500px|center]] | |[[File:LMU-Munich-integration-Picture4.png|500px|center]] | ||
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| + | |'''D''': Linearized vector is transformed into ''B. subtilis'' | ||
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|[[File:LMU-Munich-integration-Arrow.png|80px|center]] | |[[File:LMU-Munich-integration-Arrow.png|80px|center]] | ||
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|[[File:LMU-Munich-integration-Picture5.png|500px|center]] | |[[File:LMU-Munich-integration-Picture5.png|500px|center]] | ||
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| + | |'''E''': Double recombination of homologuos sequences | ||
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|[[File:LMU-Munich-integration-Picture6.png|500px|center]] | |[[File:LMU-Munich-integration-Picture6.png|500px|center]] | ||
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| + | |'''F''': Final genotype | ||
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Revision as of 10:50, 25 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Integrative vectors for Bacillus subtilis
Many vectors for B. subtilis are integrative, so are all except for one that we provide to the registry. They all insert into the genome via double homologous recombination at specific sites. A target gene in B. subtilis is disrupted which can be selected for by specific tests. These are part of our protocol "How to use Bacillus subtilis vectors".
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