Team:TU Munich/Notebook/Labjournal

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(Created page with "==Saturday, 23th June== ===Quick Change mutagenis to remove NgoMIV from pYES2=== Investigator: Ingmar, Volker")
(→Quick Change mutagenis to remove NgoMIV from pYES2)
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==Saturday, 23th June==
==Saturday, 23th June==
===Quick Change mutagenis to remove NgoMIV from pYES2===
===Quick Change mutagenis to remove NgoMIV from pYES2===
-
Investigator: Ingmar, Volker
+
'''Investigator: Ingmar, Volker'''
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 +
 
 +
{|
 +
|'''
 +
== PCR reaction batch ==
 +
'''
 +
|-
 +
|'''volume'''
 +
|'''substance'''
 +
|-
 +
|2.5 µl
 +
|10x Pfu Ultra II buffer
 +
|-
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|4 µl
 +
|P7 template
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|-
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|0.5 µl
 +
|1:10 dilution of O38 (10 pmol/µL)
 +
|-
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|0.5 µl
 +
|1:10 dilution of O39 ((10 pmol/µL)
 +
|-
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|17 µl
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|ddH2O
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|-
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|0.5 µl
 +
|dNTP mix
 +
|-
 +
|0.5 µl
 +
|Pfu Turbo DNA polymerase (2.5 U / µl)
 +
|}

Revision as of 15:27, 23 June 2012

Saturday, 23th June

Quick Change mutagenis to remove NgoMIV from pYES2

Investigator: Ingmar, Volker


PCR reaction batch

volume substance
2.5 µl 10x Pfu Ultra II buffer
4 µl P7 template
0.5 µl 1:10 dilution of O38 (10 pmol/µL)
0.5 µl 1:10 dilution of O39 ((10 pmol/µL)
17 µl ddH2O
0.5 µl dNTP mix
0.5 µl Pfu Turbo DNA polymerase (2.5 U / µl)
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