Team:EPF-Lausanne/Results

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==Part assembly==
==Part assembly==
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We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the Registry[http://partsregistry.org/Main_Page|Registry]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.
+
We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the [http://partsregistry.org/Main_Page Registry]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.
-
'''LovTAP-VP16:''' BBa_K838000 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000]
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'''LovTAP-VP16:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]
[[File:Team-EPF-Lausanne LovTAPbb.JPG|600px]]
[[File:Team-EPF-Lausanne LovTAPbb.JPG|600px]]
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'''LovTAP readout (RO):''' BBa_K838001 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001]
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'''LovTAP readout (RO):''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]
[[File:Team-EPF-Lausanne pSB1C3-RO.JPG|600px]]
[[File:Team-EPF-Lausanne pSB1C3-RO.JPG|600px]]
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'''Melanopsin:''' BBa_K838002 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002]
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'''Melanopsin:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]
[[File:Team-EPF-Lausanne melanopsinbb.JPG|600px]]
[[File:Team-EPF-Lausanne melanopsinbb.JPG|600px]]
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'''NFAT response element:''' BBa_K838003 [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003]
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'''NFAT response element:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003 BBa_K838003]
[[File:Team-EPF-Lausanne pSB1C3-NFAT.JPG|600px]]
[[File:Team-EPF-Lausanne pSB1C3-NFAT.JPG|600px]]

Revision as of 08:48, 25 September 2012

Part assembly

We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the [http://partsregistry.org/Main_Page Registry]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.

LovTAP-VP16: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]

Team-EPF-Lausanne LovTAPbb.JPG

LovTAP readout (RO): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]

Team-EPF-Lausanne pSB1C3-RO.JPG

Melanopsin: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]

Team-EPF-Lausanne melanopsinbb.JPG

NFAT response element: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003 BBa_K838003]

Team-EPF-Lausanne pSB1C3-NFAT.JPG


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