Team:LMU-Munich/Data

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Revision as of 07:38, 25 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

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Data banner.jpg

Data

Here you will find all of our project data.

BacillusBioBrickBox

Evaluation of B. subtilis vectors


Evaluation of B. subtilis vectors in B. subtilis with different inserts.

Anderson Promoter Evaluation


Evaluation of the Anderson promoters in B. subtilis with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays).

Constitutive Bacillus Promoters


Evaluation of the constitutive B. subtilis promoters P_liaG, P_veg and P_lepA with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays).

Inducible Bacillus Promoters


Evaluation of the inducible B. subtilis promoter P_liaI with the lux operon (luminescence) and the reporter lacZ (β-galactosidase assays).

Sporobeads

Spore purification


We compared different ways of purifying Bacillus spores from vegetative cells

GerminationSTOP

Knockouts of germination genes


Germination assy of Bacillus strains with multiple gene knockouts

Trashcan

Things that were a pain in the neck!

cgeA and P_cgeA


Unexpected restriction sites combined with no activity at all

MazF

The story of trying to clone a toxine from E. coli

The story of trying to clone a toxine from E. coli

For our big breakthroughs, or to follow specific projects, see the individual project pages:

Project Navigation


Bacillus Intro	Bacillus BioBrickBox	Sporobeads	Germination STOP

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Data"

@@ Line 36: / Line 36: @@
 ====Constitutive ''Bacillus'' Promoters====
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Evaluation of the constitutive ''B. subtilis'' promoters P<sub>''liaG''</sub>, P<sub>''veg''</sub> and P<sub>''lepA''</sub> with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p>
+|<p align="justify">Evaluation of the constitutive ''B. subtilis'' promoters '''P<sub>''liaG''</sub>''', '''P<sub>''veg''</sub>''' and '''P<sub>''lepA''</sub>''' with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p>
 |[[File:LMU Constitutive preview.jpg|200px|link=Team:LMU-Munich/Data/Constitutive]]
 |-
@@ Line 45: / Line 45: @@
 ====Inducible ''Bacillus'' Promoters====
 {| "width=100%" style="text-align:center;"|
-|<p align="justify">Evaluation of the inducible ''B. subtilis'' promoter P<sub>''liaI''</sub> with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p>
+|<p align="justify">Evaluation of the inducible ''B. subtilis'' promoter '''P<sub>''liaI''</sub>''' with the ''lux'' operon (luminescence) and the reporter ''lacZ'' (β-galactosidase assays).</p>
 |[[File:Englisch Auswertung PliaI.png|200px|link=Team:LMU-Munich/Data/Inducible]]
 |-