Team:Arizona State

From 2012.igem.org

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{{:Team:Arizona_State/sitemap}}
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<h1>Website To Do List:</h1>
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<p><b>DO NOT DELETE THIS LIST!</b> may be used for "Site Map". Due October 3, <b><i>LESS THAN TWO WEEKS!!!</i></b></p>
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<table>
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    <th>Project</th>
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    <th>Team</th>
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    <th>Results</th>
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    <th>Human Practices</th>
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    <th>Extras</th>
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    <tr valign="top">
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<td>
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  <ul><li>make "Home" page pretty</li>
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<li>add info to "Problem" page</li>
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<li>make "Overview" page pretty</li>
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<li>add info to "Magainin" page</li>
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<li>add info to "Chimeric Reporter" page</li>
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<li>add info to "ssDNA" page</li>
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  </ul> </td>
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<td>
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  <ul>
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        <li>update bios on "Team" page</li>
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      </ul></td>
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<td>
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  <ul>
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    <li>Get Lab Data </li>
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    <li><s>add info to "Problem" page</s></li>
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    <li>add info to "Data" page</li>
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    <li>add info to "Main Results" page</li>
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    <li>add info to "Judging Criteria" page</li>
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            <li>add info to "Regional Jamboree" page</li>
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      </ul></td>
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<td>
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  <ul><li>approve "University" page?</li>
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  <li>approve to "Community" page?</li>
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  <li><s>add info to "Regional Jamboree" page</s></li>
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  <li>approve "International" page?</li>
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  <li>add info to "Field Applications" page</li>
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  <li>add info to "Modeling" page</li>
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  </ul> </td>
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<td>
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  <ul><li>approve "Safety" page?</li>
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<li>add info to "Site Map" page</li>
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    <li>make banner/logo to put on top of page</li>
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  </ul> </td>
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</tr>
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</table>
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</html>
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Revision as of 04:17, 25 September 2012


Abstract

Diarrheic pathogens including E.coli O157:H7 serotype, campylobacter, shigella, and salmonella often contaminate drinking water supplies in developing nations and are responsible for approximately 1.5 million worldwide annual deaths. Current technologies for detection of bacteria include DNA hybridization FRET signaling, electrical detection via immobilized antimicrobial peptides, and PCR amplification followed by gel visualization. Our method of bacterial detection fills a niche in biosensor technology. Our design implies lower costs, higher portability, and a more rapid signal output than most bacterial biosensors. Additionally, our interchangeable DNA probe confers modularity, allowing for a range of bacterial detection. Using a novel split beta-galactosidase complementation assay, we have designed three unique chimeric proteins that recognize and bind to specific pathogenic markers and create a functioning beta-galactosidase enzyme. This functioning enzyme unit then cleaves x-gal and produces a colorimetric output signal. Our research demonstrates success in initial stages of chimeric protein assembly.


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