Transformation Synechocystis 6803

1. Make sure the cells are in exponential growth phase. (OD730 of 0.8)

2. Take 15 ml of cell culture and spin it down for 15 minutes at RT.

3. ReSuspend the pellet to 1ml in BG11 broth.

4. Mix in the plasmid to a final concentration of 150 ng/ml

5. Incubate for 5 h at 25 degrees C and in the light.

6. Plate the cells onto BG11 plates with antibiotic.

Spray your gloves, containers, and working surfaces with ethanol, and let the ethanol dry, before handling cyanobacteria.