Team:EPF-Lausanne/Protocol/GelExtraction

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A gel extraction is used to select a fragment of DNA of a specific length
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out of a solution composed of different fragments (ideally the difference
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in length between the wanted fragment and the closest-sized fragment should
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be more than 200bp). These fragments are often obtained after a
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[[Team:EPF-Lausanne/Protocol/Digestion|digestion]] .
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Start with a lot of DNA (at least 2 ug of DNA to get reasonable yields), and
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load a [[Team:EPF-Lausanne/Protocol/Gel|gel]]
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We used Macherey-Nagel's "Nucleospin&#174; Gel and PCR clean-up" kit.
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Its manual can be found here: [http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA%20clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf Gel and PCR clean-up Manual]
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Revision as of 22:02, 24 September 2012

Protocol: Gel Extraction


A gel extraction is used to select a fragment of DNA of a specific length out of a solution composed of different fragments (ideally the difference in length between the wanted fragment and the closest-sized fragment should be more than 200bp). These fragments are often obtained after a digestion .

Start with a lot of DNA (at least 2 ug of DNA to get reasonable yields), and load a gel

We used Macherey-Nagel's "Nucleospin® Gel and PCR clean-up" kit. Its manual can be found here: [http://www.mn-net.com/Portals/8/attachments/Redakteure_Bio/Protocols/DNA%20clean-up/UM_PCRcleanup_Gelex_NSGelPCR.pdf Gel and PCR clean-up Manual]