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Revision as of 20:49, 24 September 2012

Contents 1 September 18 1.1 Gel extraction 1.2 Ligation 1.3 Transformation 1.4 Observation through a confocal microscope 1.5 Restriction enzyme processing

September 18

Gel extraction

写真お願いします。

• LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL 1.37(260/280) 0.82(260/230)

Ligation

J23107-tatABCD-pspA-DT(EcoR1, Spe1) 14.2μg/mL	LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL	Ligation High Ver.2	total
8	1.5	9.5	19

LacP-torA-GFP-DT(EcoR1, Xba1) 36.9μg/mL	Ligation High Ver.2	total
1.5	1.5	3

at 4℃ for 1 hour

Transformation

• J23107-tatABCD-pspA-DT-LacP-torA-GFP-DT
• LacP-torA-GFP-DT(EcoR1, Xba1)

Observation through a confocal microscope

We made sample for observation through a confocal microscope We incubated sample with 0mM/0.5mM IPTG for 1.5 hours. Then, we incubated onto medium without IPTG for 2.5 hours.

IPTG(mM)	medium
0	LB
0.1	LB
0.1	LB with 0.1mM IPTG
0	LB with 2 percent glucose
0.1	LB with 2 percent glucose
0	LB with 5 percent glucose
0.1	LB with 5 percent glucose
0	LB with 2 percent glucose
0	LB ここ途中
0	LB
0	LB

Then, we incubated onto medium without IPTG for 2.5 hours.

Restriction enzyme processing

J23101-Kil-DT	BufferM	XbaI	Pst	BSA	MilliQ	total
15	3	1	1	3	7	30

J23101-Kil-DT	BufferM	XbaI	BSA	MilliQ	total
3	1	0.5	1	4.5	10

J23101-Kil-DT	BufferH	Pst	MilliQ	total
3	1	0.5	5.5	10

37℃

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