Team:TU-Delft/FUSGFP

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   <o:Company>TU Delft</o:Company>
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mso-ansi-language:EN-US;font-weight:normal;mso-bidi-font-weight:bold'><o:p></o:p></span></h1>
mso-ansi-language:EN-US;font-weight:normal;mso-bidi-font-weight:bold'><o:p></o:p></span></h1>
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<h1 style='margin-top:0cm;line-height:normal'><span lang=EN-US
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<h1 style='margin-top:0cm;line-height:normal'><span class=SpellE><span
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style='font-size:11.0pt;font-family:"Calibri","sans-serif";mso-ascii-theme-font:
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lang=EN-US style='font-size:10.0pt;font-family:"Courier New";color:windowtext;
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minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;
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background:yellow;mso-highlight:yellow;mso-ansi-language:EN-US;font-weight:
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mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
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normal;mso-bidi-font-weight:bold'>AATCAAA</span><span lang=EN-US
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style='font-size:10.0pt;font-family:"Courier New";color:windowtext;background:
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font-weight:normal'>AATCAAAGGATCC</span><span lang=EN-US style='font-size:11.0pt;
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olive;mso-highlight:olive;mso-ansi-language:EN-US;font-weight:normal;
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mso-bidi-font-weight:bold'>GGATCC</span><tt><span lang=EN-US style='font-size:
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10.0pt;mso-fareast-font-family:Calibri;mso-fareast-theme-font:minor-latin;
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color:windowtext;mso-ansi-language:EN-US;font-weight:normal'>aAaAaA</span><span
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background:lime;mso-highlight:lime;mso-ansi-language:EN-US'>aAaAaA</span></tt><span
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lang=EN-US style='font-size:11.0pt;font-family:"Calibri","sans-serif";
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lang=EN-US style='font-size:10.0pt;font-family:"Courier New";color:windowtext;
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mso-ascii-theme-font:minor-latin;mso-fareast-font-family:Calibri;mso-fareast-theme-font:
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minor-latin;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";
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mso-bidi-font-weight:bold'>ATG</span></span><span lang=EN-US style='font-size:
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font-weight:normal'>ATGGTGAGCAAGGGCGAGGAGCTGTTCACC<wbr>GGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTG<wbr>TCCGGCGAGGGCGAGGGCGATGCCACCTAC<br>
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font-weight:normal;mso-bidi-font-weight:bold'><o:p></o:p></span></h1>
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<pre><span lang=EN-US style='mso-ansi-language:EN-US'>GTGAGCAAGGGCGAGGAGCTGTTCACC<wbr>GGGGTGGTGCCCATCCTGGTCGAGCTGGAC<br>
 +
GGCGACGTAAACGGCCACAAGTTCAGCGTG<wbr>TCCGGCGAGGGCGAGGGCGATGCCACCTAC<br>
GGCAAGCTGACCCTGAAGTTCATCTGCACC<wbr>ACCGGCAAGCTGCCCGTGCCCTGGCCCACC<br>
GGCAAGCTGACCCTGAAGTTCATCTGCACC<wbr>ACCGGCAAGCTGCCCGTGCCCTGGCCCACC<br>
CTCGTGACCACCCTGACCTACGGCGTGCAG<wbr>TGCTTCAGCCGCTACCCCGACCACATGAAG<br>
CTCGTGACCACCCTGACCTACGGCGTGCAG<wbr>TGCTTCAGCCGCTACCCCGACCACATGAAG<br>
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GGCATCAAGGTGAACTTCAAGATCCGCCAC<wbr>AACATCGAGGACGGCAGCGTGCAGCTCGCC<br>
GGCATCAAGGTGAACTTCAAGATCCGCCAC<wbr>AACATCGAGGACGGCAGCGTGCAGCTCGCC<br>
GACCACTACCAGCAGAACACCCCCATCGGC<wbr>GACGGCCCCGTGCTGCTGCCCGACAACCAC<br>
GACCACTACCAGCAGAACACCCCCATCGGC<wbr>GACGGCCCCGTGCTGCTGCCCGACAACCAC<br>
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TACCTGAGCACCCAGTCCGCCCTGAGCAAA<wbr>GACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGAT<wbr>CACTCTCGGCATGGACGAGCTGTACAAG<span
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TACCTGAGCACCCAGTCCGCCCTGAGCAAA<wbr>GACCCCAACGAGAAGCGCGATCACATGGTC<br>
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style='background:red;mso-highlight:red'><wbr>TAA<wbr>TAAC</span><span
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CTGCTGGAGTTCGTGACCGCCGCCGGGATC<wbr>ACTCTCGGCATGGACGAGCTGTACAAGTAA<span
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style='background:gray;mso-highlight:gray'>ATATG</span><span style='background:
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style='background:red;mso-highlight:red'><o:p></o:p></span></span></pre><pre><span
-
fuchsia;mso-highlight:fuchsia'>taataagcgaatttcttatgatttatgatttt</span></span><span
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class=SpellE><span lang=EN-US style='background:red;mso-highlight:red;
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mso-ansi-language:EN-US'>TAATAA</span><span lang=EN-US style='background:silver;
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mso-highlight:silver;mso-ansi-language:EN-US'>CATATG</span><span lang=EN-US
 +
style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:EN-US'>taataagcgaatttcttatgatttatgatttt</span></span><span
lang=EN-US style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:
lang=EN-US style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:
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EN-US'><o:p></o:p></span></h1>
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EN-US'><o:p></o:p></span></pre><pre><span class=SpellE><span lang=EN-US
-
 
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style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:EN-US'>Tattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggt</span></span><span
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<pre><span class=SpellE><span lang=EN-US style='background:fuchsia;mso-highlight:
+
-
fuchsia;mso-ansi-language:EN-US'>Tattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggt</span></span><span
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lang=EN-US style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:
lang=EN-US style='background:fuchsia;mso-highlight:fuchsia;mso-ansi-language:
EN-US'><o:p></o:p></span></pre><pre><span class=SpellE><span lang=EN-US
EN-US'><o:p></o:p></span></pre><pre><span class=SpellE><span lang=EN-US
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style='mso-bidi-font-weight:normal'><span lang=EN-US style='background:blue;
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font-family:"Courier New";mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
 
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<p class=MsoNormal><span lang=EN-US style='mso-ansi-language:EN-US'>Total
 
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amount of <span class=SpellE>nt’s</span>: 1203<o:p></o:p></span></p>
 
<p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;line-height:115%;
<p class=MsoNormal><span lang=EN-US style='font-size:10.0pt;line-height:115%;

Latest revision as of 14:08, 24 September 2012

FUS1-YEGFP

Prefix-Promoter(FUS1)-BamHI-Kozak-Start-gene-Stop-Nde1-Terminator(ADH1)-Suffix

 

gaattcgcggccgcttctagagTTTTGGTGCGATG

ATGAAACAAACATGAAACGTCAGTAATTTGAAACAAATAACGTAATTCTCGGGATTGGTT

TTATTTAAATGACAATGTAAGAGTGGCTTTGTAAGGTATGTGTTGCTCTTAAAATATTTG

GATACGACATCCTTTATCTTTTTCCTTTAAGAGCAGGATATAAGCCATCAAGTTTCTGAA

AATCAAAGGATCCaAaAaAATG

GTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGAC

GGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTAC

GGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACC

CTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAG

CAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTC

TTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTG

GTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCAC

AAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAAC

GGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCC

GACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCAC

TACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTC

CTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA
TAATAACATATGtaataagcgaatttcttatgatttatgatttt
Tattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggt
Tttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctca
Ggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcct
gcaaatcgctccctactagtagcggccgctgcag

 

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