Team:Tsinghua-D

     By creating a term “Designable Thermoswitch”, we are trying to deliver an idea that metabolic controllers responded to given temperatures can be designed.

     Besides explanation and prediction, the ultimate goal of science is creation. Here, we create several riboswitches as thermal metabolic controller. Pre-set a “switch-on” temperature and a “switch-off” temperature, and in silico simulation will give the sequence of riboswitch that meet the requirement.

     A step further, we apply our “Designable Thermoswitches” th the field of fermentation industry. For a lone time, Engineers are trying to find a more economic and more automatic way to extract fermentation product inside the engineered microorganism.  We align our “Designable Thermoswitches” and gene of lysozyme together and put them into E.Coli to solve this problem. The reconstructed E.Coli will switch on the procedure of self-lysate at the given temperature and release the fermentation product inside.