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Team:Trieste/notebook
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<h2 class="notebook_title">Suicide System</h2> | <h2 class="notebook_title">Suicide System</h2> | ||
We prepared the antibiotics stock ( Amp, Kan, Cm, Spec ) and the DH5-alpha competent cells.</br> | We prepared the antibiotics stock ( Amp, Kan, Cm, Spec ) and the DH5-alpha competent cells.</br> | ||
| + | </br> | ||
We extracted the following BB from the kit: the constitutive promoter ( BB_J23100), the strong RBS (BB_B0034), the weak RBS (BB_B0031), the GFP (BB_E0240) the double terminator (BB_B0015), the toxin Tse2 (BB_K314200).</br> | We extracted the following BB from the kit: the constitutive promoter ( BB_J23100), the strong RBS (BB_B0034), the weak RBS (BB_B0031), the GFP (BB_E0240) the double terminator (BB_B0015), the toxin Tse2 (BB_K314200).</br> | ||
| + | </br> | ||
E.coli DH5-alpha competent cells were transformed with 2 ul of each BB extracted.</br> | E.coli DH5-alpha competent cells were transformed with 2 ul of each BB extracted.</br> | ||
| - | We learnt the safety rules and mostly of the lab techniques | + | </br> |
| + | We learnt the safety rules and mostly of the lab techniques. | ||
</div> | </div> | ||
<div id="antibody" class="notebook_section"> | <div id="antibody" class="notebook_section"> | ||
Revision as of 16:40, 23 September 2012
The Jolly JoCare
a safe probiotic platform for protein expression
Week 1
More
Suicide System
We prepared the antibiotics stock ( Amp, Kan, Cm, Spec ) and the DH5-alpha competent cells. We extracted the following BB from the kit: the constitutive promoter ( BB_J23100), the strong RBS (BB_B0034), the weak RBS (BB_B0031), the GFP (BB_E0240) the double terminator (BB_B0015), the toxin Tse2 (BB_K314200). E.coli DH5-alpha competent cells were transformed with 2 ul of each BB extracted. We learnt the safety rules and mostly of the lab techniques.Antibody
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.Chassis
Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.
