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Team:HKU HongKong/Data/Protocols.html
From 2012.igem.org
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100uL competent cells. </span></font></p> | 100uL competent cells. </span></font></p> | ||
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Incubate on ice for 10 min. </span></font></li> | Incubate on ice for 10 min. </span></font></li> | ||
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<span style="font-size: 10pt; font-family: Tahoma; font-weight: 400">Place | <span style="font-size: 10pt; font-family: Tahoma; font-weight: 400">Place | ||
in water bath at 42°C for 90s.</span></font></li> | in water bath at 42°C for 90s.</span></font></li> | ||
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Revision as of 14:11, 23 September 2012
Team:HKU HK
From 2011.igem.org
Molecular Cloning Protocols
Transforming Competent E.coli with the
Plasmid:
We chose the biobrick K137076 to
ligate to the pvdQ gene for the following reasons:
-
Take out the competent E-coli cells from the -80 freezer. (Keep all tubes on ice).
-
Add 1uL of the plasmid DNA in 100uL competent cells (if from Kit). For transformation of ligated product, add 10uL of the ligated plasmid DNA in 100uL competent cells.
- Incubate on ice for 10 min.
-
Place in water bath at 42°C for 90s.
-
Place immediately back on ice for at least 2 min
