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Team:Carnegie Mellon
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</td></tr></table><script type="text/javascript"> if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script> | </td></tr></table><script type="text/javascript"> if (window.showTocToggle) { var tocShowText = "show"; var tocHideText = "hide"; showTocToggle(); } </script> | ||
<a name="Introduction:_Motivation"></a><h2> <span class="mw-headline"> Introduction: Motivation </span></h2> | <a name="Introduction:_Motivation"></a><h2> <span class="mw-headline"> Introduction: Motivation </span></h2> | ||
| - | <ul><li> We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency and transcriptional strength. | + | <ul><li> We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure a variety of metrics in gene expression networks including translational efficiency and transcriptional strength. |
</li></ul> | </li></ul> | ||
| - | <ul><li> We believe that we can use Spinach (a fluorescent RNA sequence) and a FAP (fluorogen activating protein) as | + | <ul><li> We believe that we can use Spinach (a fluorescent RNA sequence) and a FAP (fluorogen activating protein) as biosensors to reflect these metrics <i>in vivo</i>, rather than <i>in vitro</i>, which has previously proven to be very costly and impractical. |
</li></ul> | </li></ul> | ||
<ul><li> We will characterize the relationship between genetic expression of Spinach (upstream), a FAP (downstream), translational efficiency, and transcriptional strength.<br /> | <ul><li> We will characterize the relationship between genetic expression of Spinach (upstream), a FAP (downstream), translational efficiency, and transcriptional strength.<br /> | ||
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</li><li> Determining promoter strength <i> in vivo</i> | </li><li> Determining promoter strength <i> in vivo</i> | ||
</li><li> <i>in vivo</i> mRNA and protein half-lives | </li><li> <i>in vivo</i> mRNA and protein half-lives | ||
| + | </li><li> Introducing a protein reporter that has virtually no maturation rate and is limited only by the very quick absorption rate of the fluorogen into the cell | ||
| + | </li><li> Introducing a functioning mRNA reporter | ||
| + | </li><li> Providing a method to better characterize current and future BioBricks | ||
</li></ol> | </li></ol> | ||
<p>Our proposed BioBrick is novel, and potentially very useful in practice. | <p>Our proposed BioBrick is novel, and potentially very useful in practice. | ||
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</li><li> Programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for modeling our BioBrick to students, | </li><li> Programmed <a href="https://2012.igem.org/Team:Carnegie_Mellon/Software" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Software" rel="nofollow">a new piece of software</a> for modeling our BioBrick to students, | ||
</li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">Developed and tested techniques for measuring translational efficiency and transcriptional strength, | </li><li> <a href="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" class="external text" title="https://2012.igem.org/Team:Carnegie_Mellon/Protocols" rel="nofollow">Developed and tested techniques for measuring translational efficiency and transcriptional strength, | ||
| - | </li><li> Participated in human practices demonstration | + | </li><li> Participated in human practices demonstration and modeled our biological system using a programmable and interactive, electrical analog. |
</li></ul> | </li></ul> | ||
Revision as of 15:44, 19 June 2012
Welcome to Carnegie Mellon University 2012 iGEM Team Wiki!
Contents |
Introduction: Motivation
- We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure a variety of metrics in gene expression networks including translational efficiency and transcriptional strength.
- We believe that we can use Spinach (a fluorescent RNA sequence) and a FAP (fluorogen activating protein) as biosensors to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
- We will characterize the relationship between genetic expression of Spinach (upstream), a FAP (downstream), translational efficiency, and transcriptional strength.
Abstract/Introduction
Motivation question
Humanistic implications go here
Primary Objective: A Useful BioBrick for Synthetic Biologists
We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:
- Quantifying translational efficiency in vivo
- Troubleshooting in expression strains
- mRNA and protein localization
- in vivo transcription rate analysis
- Determining promoter strength in vivo
- in vivo mRNA and protein half-lives
- Introducing a protein reporter that has virtually no maturation rate and is limited only by the very quick absorption rate of the fluorogen into the cell
- Introducing a functioning mRNA reporter
- Providing a method to better characterize current and future BioBricks
Our proposed BioBrick is novel, and potentially very useful in practice.
Secondary Objective: Humanistic Practice
FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).
Further Considerations
In the pursuit of our project, as well as the biological aspects, we:
- Considered aspects of scale-up, including the ethical, legal and social implications of our BioBrick,
- Programmed a new piece of software for modeling our BioBrick to students,
- Developed and tested techniques for measuring translational efficiency and transcriptional strength,
- Participated in human practices demonstration and modeled our biological system using a programmable and interactive, electrical analog.
