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Team:SDU-Denmark/labwork/Notebook/week4
From 2012.igem.org
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We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on. <br/> | We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on. <br/> | ||
| - | We ran a gel on the samples and found that | + | We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector. |
These will be shipped of to be sequneced tomorrow. | These will be shipped of to be sequneced tomorrow. | ||
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| - | <p> <b> | + | <p> <b>25-07-2012:</b> </p> |
| - | <h2>SST send for sequencing </h2> <br/> | + | <h2> SST send for sequencing </h2> <br/> |
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| - | + | The two sample from the day before were prepared for sequencing by diluting them to 70-78 ng/μl and sent them for sequencing. Afterward we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing. | |
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Revision as of 13:29, 23 September 2012
Laboratory Notebook
Here you find the log book for the procedures carried out in the laboratory, starting from week 27.
23-07-2012:
SST send for sequencing
SST from the gel extraction the 21-07-2012 was diluted to a concentration of 100ng/µL and separated to 4 samples of 15µL each and send of for sequencing.
We made liquid cultures from the SST plates from 19-07-2012 and incubated them O.N.
24-07-2012:
Cryo backup, miniprep and preparation to sequencing
We made some Cryo backup’s from the 18 liquid cultures, that was incubated O.N. and took some sample out from each to do a miniprep on.
We ran a gel on the samples and found that two samples had bands corresponding to our cut gene and vector.
These will be shipped of to be sequneced tomorrow.
25-07-2012:
SST send for sequencing
The two sample from the day before were prepared for sequencing by diluting them to 70-78 ng/μl and sent them for sequencing. Afterward we looked into the expected outcomes of sequencing and had a look at the following mutagenesis post-sequencing.
