Team:NRP-UEA-Norwich/TheoreticalProjects
From 2012.igem.org
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There are four central mathematical operations; subtraction, multiplication, addition and subtraction; the ability to do each author is in a cell unleashes massive potential for future applications. | There are four central mathematical operations; subtraction, multiplication, addition and subtraction; the ability to do each author is in a cell unleashes massive potential for future applications. | ||
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+ | [[File:IGEM_attenuation_diagram_1_12.09.23.png | 250px | right]] | ||
A multiplier effect can be produced by using three loop system that causes attenuation in the tryptophan operon. The removal of the ribosome binding site up stream means the formation of the stem and loops are no longer dependent on the ribosome. Instead the team designed a synthetic gene that would produce an arm and a that would complimentary bind to cite one in their leader causing the sight three four loop to form and transcription to be attenuated. The probability of this happening is dependent on the concentration of the complimentary RNAs but also on the transcription initiation rate all the promoter creating a multiplier effect (where the rate of transcription initiation in the promoter is multiplied by a number between nought and one) | A multiplier effect can be produced by using three loop system that causes attenuation in the tryptophan operon. The removal of the ribosome binding site up stream means the formation of the stem and loops are no longer dependent on the ribosome. Instead the team designed a synthetic gene that would produce an arm and a that would complimentary bind to cite one in their leader causing the sight three four loop to form and transcription to be attenuated. The probability of this happening is dependent on the concentration of the complimentary RNAs but also on the transcription initiation rate all the promoter creating a multiplier effect (where the rate of transcription initiation in the promoter is multiplied by a number between nought and one) | ||
Revision as of 11:40, 23 September 2012