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Team:TU Darmstadt/Project/Metabolism/Materials
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==Chemicals== | ==Chemicals== | ||
Revision as of 10:27, 23 September 2012
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Protocols Metabolism
Contents |
Chemicals
| No. | Name | Firma | Order-ID |
| 1 | Terephthalat | Sigma-Aldrich | 185361-500G |
| 2 | Acetic acid | Roth | 6755.1 |
| 3 | Methanol | Roth | CP43.1 |
| 4 | Coomassie Brilliant Blue R-250 | Roth | 9598.1 |
| 5 | Trypton | Roth | 8952.1 |
| 6 | Ethylene glycol | Roth | 9516.1 |
| 7 | 3,4-Dihydroxybenzoic acid | Sigma-Aldrich | 37580-25G-F |
| 8 | Catechol | Sigma-Aldrich | C9510-100G |
| 9 | Isopropyl β-D-1-thiogalactopyranoside | Roth | CN08.1 |
| 10 | Tetramethylethylenediamine | Roth | 2367.3 |
| 11 | Ammonium persulfate | Roth | 9592.3 |
| 12 | Bromophenol blue | Roth | A512.1 |
| 13 | Glucose | Roth | X997.1 |
| 14 | Calcium chloride | Roth | CN92.1 |
| 15 | Dithiothreitol | Roth | 6908.1 |
| 16 | Sodium carbonate | Roth | 8563.1 |
| 17 | Phenylmethanesulfonylfluoride | Roth | 6367.1 |
| 18 | NADH-Disodium | Roth | AE12.1 |
| 19 | Nicotinamide adenine dinucleotide | Roth | AE11.1 |
| 20 | orthophosphoric acid 85% | Roth | 9079.1 |
| 21 | Trichloroacetic acid | Roth | 8789.2 |
| 22 | Quick-Load® 100 bp DNA Ladder | NEB Biolabs | N0467S |
| 23 | Phenol | AppliChem | A4458.0250 |
| 24 | Dimethyl sulfoxide | AppliChem | A1584.0100 |
| 25 | Methylene blue | AppliChem | A4084.0025 |
| 26 | Magnesium sulfate | AppliChem | A6287.0250 |
| 27 | Cetrimonium bromide | AppliChem | A0805.0100 |
| 28 | Tetramethylethylenediamine | AppliChem | A1148.0025 |
| 29 | Urea | AppliChem | A5470.0500 |
| 30 | Tween20 | AppliChem | A1389.0500 |
| 31 | Protein Marker III (6,5-200) | AppliChem | A4402.0001 |
| 32 | Thiamine hydrochloride | AppliChem | A09955.0050 |
| 33 | Triton X-100 | AppliChem | A1388.0500 |
| 34 | DNA-Dye NonTox | AppliChem | A9555.1000 |
| 35 | Agarose | Roth | 3810.2 |
| 36 | Ethanol 96 % | Roth | T171.4 |
| 37 | Ethylenediaminetetraacetic acid | Roth | X986.2 |
| 38 | Ethanol | Roth | P076.1 |
| 39 | Tetracycline hydrochloride | Roth | 237.1 |
| 40 | D-Desthiobiotin 10x Buffer E | iba | 2-1000-025 |
| 41 | 2-(4-Hydroxyphenylazo)benzoic acid | Sigma-Aldrich | H5126-5G |
| 42 | 10 mM dNTP-Mix | Roth | L785.2 |
| 43 | Hydrochloric acid | Roth | 9277.1 |
| 44 | Acrylamid - Bis (37,5:1) | Roth | 3029.1 |
| 45 | Ammonium acetate | Roth | 7869.2 |
| 46 | Agar-Agar | Roth | 5210.3 |
| 47 | Chloroform | Roth | 4423.1 |
| 48 | Glycine | Roth | 3790.2 |
| 49 | Dipotassium phosphate | Roth | T875.1 |
| 50 | Sodium chloride | Roth | 9265.1 |
| 51 | Yeast extract | Roth | 2363.2 |
| 52 | Monopotassium phosphate | Roth | P018.1 |
| 53 | Glycerine | Roth | 3783.1 |
| 54 | Sodium hydroxide | Roth | P031.1 |
| 55 | Tris-Base | Roth | AE15.2 |
| 56 | Isopropyl alcohol | Roth | CP41.3 |
| 57 | Isoamyl alcohol | Roth | T870.1 |
| 58 | Acetonitrile | Roth | 7330.2 |
| 59 | Acetone | Roth | 5025.5 |
| 60 | Chloramphenicol | Roth | 3886.2 |
| 61 | Ampicillin | Roth | K029.1 |
| 62 | Kanamycin | Roth | T832.1 |
| 63 | Sodium dodecyl sulfate | Roth | 5136.1 |
Primer
| No. | Name | 5´-->3´ |
| 1 | tphA2-l-F | TCATGCTTGCATCTCCTGTC |
| 2 | tphA2-l-Prefix | GAATTCGCGGCCGCTTCTAGATGCAAGAATCCATCATCCAGTGGC |
| 3 | tphA1-Suffix_R | CTGCAGCGGCCGCTACTAGTACTAATGGTTGCCAGTCGGGTCTG |
| 4 | tphA3-Suffix_R | CTGCAGCGGCCGCTACTAGTATCATAGCGGCAATGACATCAGCGTG |
| 5 | tphA3-Prefix_F | GAATTCGCGGCCGCTTCTAGATGATCCATGAAATTCAAATCGCGG |
| 6 | tphA1-l-R | CTAATGGTTGCCAGTCGGGT |
| 7 | tphA1-l-PstI(99)-F | CATGGTCTCCTCCAGGCCGGCATCGAGCT |
| 8 | tphA1-l-Prefix | GAATTCGCGGCCGCTTCTAGATGAACCACCAGATCCATATCCACG |
| 9 | tphA3-l-F | TCATAGCGGCAATGACATCA |
| 10 | tphA3-l-Prefix | GAATTCGCGGCCGCTTCTAGAGATGATCCATGAAATTCAAATCGC |
| 11 | Prefix | GAATTCGCGGCCGCTTCTAGAG |
| 12 | tphB-l-F | TCAGACCGGTTGGGCTCCGA |
| 13 | tphB-l-Prefix | GAATTCGCGGCCGCTTCTAGAGATGACAATAGTGCACCGTAGATT |
| 14 | tphA2-l-R | ATGCAAGAATCCATCATCCAG |
| 15 | tphA1-l-R | ATGAACCACCAGATCCATATC |
| 16 | tphA1-l-PstI(99)-R | CATGGTCTCCTGGAGGGCTGCATCCAGTAC |
| 17 | tphA3-l-R | ATGATCCATGAAATTCAAAT |
| 18 | Suffix | CTGCAGCGGCCGCTACTAGTA |
| 19 | tphB-l-Suffix-R | CTGCAGCGGCCGCTACTAGTATCAGACCGGTTGGGCTCCGAGTA |
| 20 | EcoRIGFxa-tphA1 | TTCTGGAATTCGGGTATTGAAGGAAGAATGAACCACCAGATCCATAT |
| 21 | EcoRIGFxa-tphA2 | TTCTGGAATTCGGGTATTGAAGGAAGAATGCAAGAATCCATCATCCA |
| 22 | EcoRIGFxa-tphA3 | TTCTGGAATTCGGGTATTGAAGGAAGAATGATCCATGAAATTCAAAT |
| 23 | EcoRIGFxa-tphB | TTCTGGAATTCGGGTATTGAAGGAAGAATGACAATAGTGCACCGTAG |
| 24 | EcoRIGFxa-aroY | TTCTGGAATTCGGGTATTGAAGGAAGAATGACAGCGCCTATCCAG |
| 25 | RBS-tphA1 | TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGAACCACCAGATCCATAT |
| 26 | RBS-tphA2 | TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGCAAGAATCCATCATCCA |
| 27 | RBS-tphA3 | TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGATCCATGAAATTCAAAT |
| 28 | RBS-tphB | TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGACAATAGTGCACCGTAG |
| 29 | RBS-aroY | TTCTGGAATTCGCGGCCGCTTCTAGAGAAAGACAGGACCTACTAGATGACAGCGCCTATCCAG |
| 30 | VF2 | TGCCACCTGACGTCTAAGAA |
| 31 | VR | ATTACCGCCTTTGAGTGAGC |
| 32 | pPR-IBA2 rev | TAGTTATTGCTCAGCGGTGG |
| 33 | pPR-IBA2 for | TAATACGACTCACTATAGGG |
Plasmids
| No. | Name | Sequence |
| 1 | pSB1C3 | [http://partsregistry.org/Part:pSB1C3 Get] |
| 2 | pSB1AK3 | [http://partsregistry.org/Part:pSB1AK3 Get] |
| 3 | BBa_J61002 | [http://partsregistry.org/Part:BBa_J61002 Get] |
| 4 | pSB1A2 | [http://partsregistry.org/Part:pSB1A2 Get] |
| 5 | pPR-IBA2 | [http://www.iba-lifesciences.com/isotope/2/2-1391-000-Sequence-pPR-IBA2.txt Get] |
Biobricks from the registry
| No. | Name | Platte | Well | Resistence | Plasmid backbone |
| 1 | BBa_K316003 | 4 | 15C | C | pSB1C3 |
| 2 | BBa_J23100 | 1 | 18C | A | BBa_J61002 |
| 3 | BBa_B0015 | 1 | 23L | A und K | pSB1AK3 |
| 4 | BBa_J61101 | 1 | 5L | A | pSB1A2 |
Bacteria
| Species | Strain | Genotype | DSM-No. |
| Escherichia coli | DH5α | F- endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15 Δ(lacZYA-argF)U169, hsdR17(rK- mK+), λ– | 6897 |
| Escherichia coli | MG1655 | F- λ- ilvG- rfb-50 rph-1 | 18039 |
| Escherichia coli | BL21(DE3)pLysS | F- ompT gal dcm lon hsdSB(rB- mB-) λ(DE3) pLysS(cmR) | none |
| Comamonas testosteroni | Kf-1 | - | 14576 |
