Team:Paris Bettencourt/Semantic containment

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Overview

We want to prevent our genetic construct from conferring an advantage to other organisms. Horizontal gene transfer (HGT) can be performed either by conjugation, transduction, or transformation. As these processes involve two parties, our genetically modified bacteria and some wild type population, and as we will not modify wild type populations, we cannot assume that HGT is fully avoidable. Semantic containment [1] means that our bacteria won't be able to "speak" with other organisms, since they don't speak the same "language", the language being DNA. Our system will read the stop codon TAG as the amino-acid serine. It means that in our bacteria the stop codon will be translated into a serine, whereas in wild type bacteria this protein will be truncated and will not confer an advantage to these cells.

Objectives

Here we want to show that semantic containment works as expected. We first create a biobrick with a tRNA Amber suppressor ([http://partsregistry.org/Part:BBa_K914000 BBa_K914000])

Design

Present the design of your system, both in a written form, and a schematic one.

Experiments and results

Characterisation of X

Experimental setup

Describe the experiment

Results

Present your results

Testing of the system

Experimental setup

Describe the experiment

Results

Present your results