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Talk:Team:Columbia-Cooper-NYC/trial
From 2012.igem.org
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div> | <div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div> | ||
</div> | </div> | ||
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| + | </p><p><br /> | ||
| + | Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for <a href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>biosensors</b></a> and as <a href="https://2010.igem.org/Team:Cambridge/Tools/Lighting" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Lighting" rel="nofollow"><b>natural light sources</b></a>. We also developed software tools to aid construction of BioBrick parts and devices. | ||
| + | </p><p><h2 class="noline" style="margin-top:30px;">Project Firefly</h2> | ||
| + | </p><p>We adopted a number of strategies to extend the use of <b>firefly luciferase</b>: | ||
| + | </p> | ||
| + | <ul><li> <a href="https://2010.igem.org/Team:Cambridge/Codons" class="external text" title="https://2010.igem.org/Team:Cambridge/Codons" rel="nofollow"><b>Codon optimisation</b></a> for increased light output | ||
| + | </li><li> Use of a <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration" rel="nofollow"><b>luciferin regenerating enzyme</b></a>. | ||
| + | </li><li> Mutagenesis to create a number of <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/Colour" rel="nofollow"><b>different colours</b></a> | ||
| + | </li></ul> | ||
| + | <p><h2 class="noline" style="margin-top:30px;">Project Vibrio</h2> | ||
| + | We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from <b>Vibrio fischeri</b>. We believe we have created the <a href="https://2010.igem.org/Team:Cambridge/Bioluminescence/G28" class="external text" title="https://2010.igem.org/Team:Cambridge/Bioluminescence/G28" rel="nofollow"><b>first BioBrick</b></a> to emit light in normal E. coli strains without the addition of any external substrate. | ||
| + | </p><p><h2 class="noline" style="margin-top:30px;">Tools</h2> | ||
| + | </p><p>During our project we made extensive use of <a href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a> to manufacture our parts, and have submitted an <a href="https://2010.igem.org/Team:Cambridge/Gibson/RFC" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/RFC" rel="nofollow"><b>RFC</b></a> to the <a href="http://bbf.openwetware.org/" class="external text" title="http://bbf.openwetware.org/" rel="nofollow"><b>BioBricks Foundation</b></a> to help future teams make best use of this technique. | ||
| + | </p><p>Along with this, we also constructed a number of tools to assist the synthetic biologists of the future: | ||
| + | </p> | ||
| + | <ul><li> <a href="https://2010.igem.org/Team:Cambridge/Tools/Gibson" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Gibson" rel="nofollow"><b>Gibthon Construct Designer</b></a> allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for <a href="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" class="external text" title="https://2010.igem.org/Team:Cambridge/Gibson/Introduction" rel="nofollow"><b>Gibson Assembly</b></a>. | ||
| + | </li><li> <a href="https://2010.igem.org/Team:Cambridge/Tools/GenBank" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/GenBank" rel="nofollow"><b>BioBrick → GenBank</b></a> allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software. | ||
| + | </li><li> The <a href="https://2010.igem.org/Team:Cambridge/Tools/Ligate" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Ligate" rel="nofollow"><b>Ligation Calculator</b></a> is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units. | ||
| + | </li><li> The <a href="https://2010.igem.org/Team:Cambridge/Tools/Eglometer" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/Eglometer" rel="nofollow"><b>E.glometer</b></a> is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in <a href="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" class="external text" title="https://2010.igem.org/Team:Cambridge/Tools/microMeasure" rel="nofollow"><b>quantitative biosensors</b></a>. | ||
| + | </li></ul> | ||
| + | <p><h2 class="noline" style="margin-top:30px;">Achievements in iGEM competition</h2> | ||
| + | </p> | ||
| + | <ul><li> Finalist | ||
| + | </li><li> Winner of "Best Wiki" award (awarded jointly to Cambridge and <a href="https://2010.igem.org/Team:Imperial_College_London" class="external text" title="https://2010.igem.org/Team:Imperial_College_London" rel="nofollow"><b>Imperial College London</b></a>) | ||
| + | </li><li> Winner of the iGEMers Prize (awarded jointly to five iGEM teams) | ||
| + | </li><li> Awarded a Gold Medal | ||
| + | </li></ul> | ||
| + | <p>Please see <a href="http://https://igem.org/Results" class="external text" title="http://https://igem.org/Results" rel="nofollow"><b>iGEM official results page</b></a> to see how all the teams did. | ||
| + | </p><p><h2 class="noline" style="margin-top:30px;">In the news</h2> | ||
| + | </p> | ||
| + | <ul><li> <a href="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" class="external text" title="http://www.newscientist.com/article/mg20827885.000-glowing-trees-could-light-up-city-streets.html" rel="nofollow">New Scientist article</a> | ||
| + | </li><li> <a href="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" class="external text" title="http://www.dailymail.co.uk/sciencetech/article-1333334/How-trees-glow-like-fireflies-day-replace-streetlights.html" rel="nofollow">Daily Mail article</a> | ||
| + | </li><li> <a href="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" class="external text" title="http://www.telegraph.co.uk/topics/christmas/8215302/The-science-of-Christmas-we-could-grow-our-own-fairy-lights-say-the-tree-wise-men.html" rel="nofollow">The Telegraph Article</a> | ||
| + | </li></ul> | ||
| + | <div class="center"><div class="thumb tnone"><div class="thumbinner" style="width:702px;"><a href="/Image:Cambridge_team_pictwo2010.jpg" class="image" title="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins"><img alt="The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins" src="/wiki/images/b/b0/Cambridge_team_pictwo2010.jpg" width="700" height="440" border="0" class="thumbimage" /></a> <div class="thumbcaption">The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins</div></div></div></div> | ||
| + | <p></div> | ||
| + | </div> | ||
| + | </div> | ||
| + | |||
</p> | </p> | ||
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Revision as of 16:13, 22 September 2012
Team:Cambridge
From 2010.igem.org
Abstract
We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.
Multimedia
If you want a break from hard-core science, check out our Gibson Assembly music video.
You can also see view videos of our bacterial bubble lamp and project overview.
Tools
The Project
A flask lit by E. coli transformed with one of our constructs
E. coli transformed with our different coloured bioluminescent systems
Team members illuminated only by our bacteria
Over the course of the summer we built a set of BioBricks to allow bioluminescence in a wide range of colours which have applications both as reporters for biosensors and as natural light sources. We also developed software tools to aid construction of BioBrick parts and devices.
Project Firefly
We adopted a number of strategies to extend the use of firefly luciferase:
- Codon optimisation for increased light output
- Use of a luciferin regenerating enzyme.
- Mutagenesis to create a number of different colours
Project Vibrio
We complemented these firefly systems, which require the addition of the substrate luciferin, with light producing systems from Vibrio fischeri. We believe we have created the first BioBrick to emit light in normal E. coli strains without the addition of any external substrate.Tools
During our project we made extensive use of Gibson Assembly to manufacture our parts, and have submitted an RFC to the BioBricks Foundation to help future teams make best use of this technique.
Along with this, we also constructed a number of tools to assist the synthetic biologists of the future:
- Gibthon Construct Designer allows the user to enter a series of BioBrick or GenBank IDs in a specific order and computes the appropriate primers for Gibson Assembly.
- BioBrick → GenBank allows parts from the registry to be downloaded in .gb format, making them compatible with a wide range of biological software.
- The Ligation Calculator is a small calculator to help you work out the proportions to use for ligation in BioBrick assembly without having to worry about units.
- The E.glometer is a cheap, easily built, piece of electronics for measuring bioluminescence. It allows scientists without access to expensive plate readers to measure bacterial light output and has potential applications in quantitative biosensors.
Achievements in iGEM competition
- Finalist
- Winner of "Best Wiki" award (awarded jointly to Cambridge and Imperial College London)
- Winner of the iGEMers Prize (awarded jointly to five iGEM teams)
- Awarded a Gold Medal
Please see iGEM official results page to see how all the teams did.
In the news
The team - in order - Anja Hohmann, Emily Knott, Hannah Copley, Will Handley, Theo Sanderson, Ben Reeve, Paul Masset, Peter Emmrich, Bill Collins
