Talk:Team:Columbia-Cooper-NYC/trial
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+ | <ul id="accmenu"> | ||
+ | <li><a class="head" href="#"> Introduction</a> | ||
+ | <ul class="llmenu llmenublue"> | ||
+ | <li><a href="/Team:Cambridge">Home</a></li> | ||
+ | |||
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+ | <li><a href="/Team:Cambridge/TheTeam">Meet the Team</a></li> | ||
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+ | </ul> | ||
+ | </li> | ||
+ | <li><a class="head" href="#">Project Firefly</a> | ||
+ | <ul class="llmenu llmenugreen"> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence">Introduction</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Background_Firefly">Background</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Colour">Coloured outputs</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Luciferin_Regeneration">Luciferin recovery</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Firefly_Modelling">Modelling</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Firefly_Characterisation">Characterisation</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li><a class="head" href="#">Project Vibrio</a> | ||
+ | <ul class="llmenu llmenublue"> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Luciferases">Introduction</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Background">Background</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/G28">The LuxBrick</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Bacterial_Codon_optimisation">Codon optimisation</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Modelling">Modelling</a></li> | ||
+ | <li><a href="/Team:Cambridge/Bioluminescence/Vibrio_Characterisation">Characterisation</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a class="head" href="#">Tools</a> | ||
+ | <ul class="llmenu llmenured"> | ||
+ | <li><a href="/Team:Cambridge/Tools/Gibson">Gibthon</a></li> | ||
+ | <li><a href="/Team:Cambridge/Tools/GenBank">BioBrick → GenBank</a></li> | ||
+ | <li><a href="/Team:Cambridge/Tools/Ligate">Ligation Calculator</a></li> | ||
+ | <li><a href="/Team:Cambridge/Tools/Eglometer">The E.glometer</a></li> | ||
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+ | <li><a class="head" href="#">Gibson Assembly</a> | ||
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+ | <li><a href="/Team:Cambridge/Gibson/Introduction">Introduction</a></li> | ||
+ | <li><a href="/Team:Cambridge/Gibson/Mechanism">How it works</a></li> | ||
+ | <li><a href="/Team:Cambridge/Gibson/Protocol">Protocol</a></li> | ||
+ | <li><a href="/Team:Cambridge/Gibson/RFC">RFC</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li><a class="head" href="#">Considerations</a> | ||
+ | <ul class="llmenu llmenublue"> | ||
+ | <li><a href="/Team:Cambridge/Human_Practices">Human Practices</a></li> | ||
+ | <li><a href="/Team:Cambridge/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | |||
+ | <li><a class="head" href="#">Future applications</a> | ||
+ | <ul class="llmenu llmenured"> | ||
+ | <li><a href="/Team:Cambridge/Tools/microMeasure">Biosensors</a></li> | ||
+ | <li><a href="/Team:Cambridge/Tools/Lighting">Lighting</a></li> | ||
+ | </ul> | ||
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+ | |||
+ | |||
+ | <li><a class="head" href="/Team:Cambridge/Notebook/Week1">Notebook</a> | ||
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+ | <li><a href="/Team:Cambridge/Notebook/Week1">Week 1</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week2">Week 2</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week3">Week 3</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week4">Week 4</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week5">Week 5</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week6">Week 6</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week7">Week 7</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week8">Week 8</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week9">Week 9</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week10">Week 10</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week11">Week 11</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/Week12">Week 12</a></li> | ||
+ | <li><a href="/Team:Cambridge/Notebook/FurtherWork">Beyond Week 12</a></li> | ||
+ | |||
+ | <li><a href="/Team:Cambridge/Protocols">Protocols</a> </li> | ||
+ | </ul> | ||
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+ | </p> | ||
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<div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div> | <div style="position:absolute; width:220px; left:485px; text-align:center;">Team members illuminated only by our bacteria</div> | ||
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Revision as of 16:12, 22 September 2012
Team:Cambridge
From 2010.igem.org
Abstract
We placed genes from fireflies and bioluminescent bacteria into E.coli. Codon optimisation and single amino acid mutagenesis allowed us to generate bright light output in a range of different colours. Future applications include and quantitative biosensors and biological alternatives to conventional lighting.
Multimedia
If you want a break from hard-core science, check out our Gibson Assembly music video.
You can also see view videos of our bacterial bubble lamp and project overview.
Tools
The Project
A flask lit by E. coli transformed with one of our constructs
E. coli transformed with our different coloured bioluminescent systems
Team members illuminated only by our bacteria