Team:Columbia-Cooper-NYC/Primers
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(Created page with "= Preparation of Primer Stock Protocol = *# Add 20x primer weight of deionized water to concentrated primer *# Vortex 20 seconds of sample with concentrated primer and deionized...")
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(Created page with "= Preparation of Primer Stock Protocol = *# Add 20x primer weight of deionized water to concentrated primer *# Vortex 20 seconds of sample with concentrated primer and deionized...")
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Revision as of 15:37, 22 September 2012
Preparation of Primer Stock Protocol
- Add 20x primer weight of deionized water to concentrated primer
- Vortex 20 seconds of sample with concentrated primer and deionized water
- Centrifuge for 10 seconds of the sample
- Diluted sample 100x again for use as stock