Team:WashU/Protocols/PCR

From 2012.igem.org

(Difference between revisions)
Line 6: Line 6:
##1uL Template
##1uL Template
##1uL 25mM dNTP's
##1uL 25mM dNTP's
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##10uL Phusion HF Buffer
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##10uL Buffer
##0.5uL Forward Primer
##0.5uL Forward Primer
##0.5uL Reverse Primer
##0.5uL Reverse Primer
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##1 cycle 94 degrees (3 minutes)
##1 cycle 94 degrees (3 minutes)
##40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
##40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)
 +
 +
==Source==
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Thanks to UW iGEM '11 for the above instructions.  The original source can be found here: [https://2011.igem.org/Team:Washington/Protocols/gel_electrophoresis University Washington gel electrophoresis protocol]

Revision as of 17:12, 18 June 2012


General PCR Protocol

  1. Set up PCR reaction tube
    1. 1uL Template
    2. 1uL 25mM dNTP's
    3. 10uL Buffer
    4. 0.5uL Forward Primer
    5. 0.5uL Reverse Primer
    6. 0.5uL TAQ polymerase
    7. 36.5uL diH2O
  1. Amplification reaction in thermocycler
    1. 1 cycle 94 degrees (3 minutes)
    2. 40 cycles 94 degrees (1 minute), 50 degrees (1 minute), 72 degrees (1 minute)

Source

Thanks to UW iGEM '11 for the above instructions. The original source can be found here: University Washington gel electrophoresis protocol

Retrieved from "http://2012.igem.org/Team:WashU/Protocols/PCR"