Team:SEU O China/Project

From 2012.igem.org

(Difference between revisions)
Line 12: Line 12:
<div id="col_nav">
<div id="col_nav">
     <div class="col_list">
     <div class="col_list">
-
         <h2>Navigation</h2>
+
         <h2>Overview</h2>
 +
<ul>
 +
    <li id="ov">Background</li>
 +
    <li id="st">Story</li>
 +
    <li id="sy">System</li>
 +
</ul>
 +
        <h2>Parts</h2>
<ul>
<ul>
-
    <li id="ov">Overview</li>
 
             <li id="li">Light Sensor</li>
             <li id="li">Light Sensor</li>
             <li id="sw">Toggle Switch</li>
             <li id="sw">Toggle Switch</li>
Line 28: Line 33:
<div class="content" id="ovcontent">
<div class="content" id="ovcontent">
-
<h3>Overview</h3>
+
<h3>Background</h3>
</html>
</html>
====Idea====
====Idea====
Line 45: Line 50:
That is to say, the biobrick system we are constructing is meant to make the single cell bacteria grow into a colony which can act like a multicellular organism. To perform gene manipulation on a multicellular level would be a promising and interesting task.  
That is to say, the biobrick system we are constructing is meant to make the single cell bacteria grow into a colony which can act like a multicellular organism. To perform gene manipulation on a multicellular level would be a promising and interesting task.  
-
====System====
 
-
In order to construct a multicellular system, several vital parts must be taken into consideration. We use a toggle switch to save a status, which simulates the cell differentiation, a AHL generate-receive system for intercelluar communication, an antisense mRNA biobrick as cell division inhibitor, in order to control the shape of bacteria colony. We designed a cell movement control device to start the differentiation automatically, as well as a light sensor system to start it artificially for easier control and further application.
+
<html>
 +
<div class="clear"></div>
 +
</div>
-
With these four parts, the story begins here:
+
<div class="content" id="stcontent">
 +
<h3>Story</h3>
 +
</html>
 +
The story begins here:
[[File:SeuoSt1.JPG |200px| center]]
[[File:SeuoSt1.JPG |200px| center]]
Line 65: Line 74:
<html>
<html>
 +
 +
<div class="clear"></div>
 +
</div>
 +
 +
<div class="content" id="sycontent">
 +
 +
<h3>System</h3>
 +
</html>
 +
 +
In order to construct a multicellular system, several vital parts must be taken into consideration. We use a toggle switch to save a status, which simulates the cell differentiation, a AHL generate-receive system for intercelluar communication, an antisense mRNA biobrick as cell division inhibitor, in order to control the shape of bacteria colony. We designed a cell movement control device to start the differentiation automatically, as well as a light sensor system to start it artificially for easier control and further application.
 +
 +
<html>
 +
<div class="clear"></div>
<div class="clear"></div>
</div>
</div>

Revision as of 10:28, 22 September 2012

header1
header2


Project





Overview

  • Background
  • Story
  • System

Parts

  • Light Sensor
  • Toggle Switch
  • Intercelluar Communication
  • Cell Division Inhibitor

Background

Idea

The original idea of this project is really simple. Getting bored of the indifferent, round round colony on the culture dish, we wish to make the bacteria grow into the form of a star, a smiling face, or any other patterns we desire.

Instead of the traditional method of painting a star on the culture dish, and let the colony grow into this pattern, we try to design a biobrick system which let the bacteria take the irregular shape all by itself, despite external conditions.

That means, not only the colony can grow into to shape of a star, but also, even if you pick one cell from the colony, and incubate it on another clean culture medium. That cell will also grow into a colony, which has the shape of a star.


Meaning

Although the idea is like some kind of bio-game, our project is not that meaningless. Comparing the single cell organism like Escherichia coli, and some simplest multicellular organism like Spongy, we notice that a significant difference between them is that the cells of a multicellular organism can act as a whole, and construct a certain structure.

That is to say, the biobrick system we are constructing is meant to make the single cell bacteria grow into a colony which can act like a multicellular organism. To perform gene manipulation on a multicellular level would be a promising and interesting task.


Story

The story begins here:

SeuoSt1.JPG

At first, the cells in a colony are all indifferent, and the toggle switches in cell are on the same status.

SeuoSt2.JPG

After some events, some of the cells become special. The switch is turned on, and the differentiation happens.

St3.JPG

The special cells generate signal which affect the nearby cells in the colony.


System

In order to construct a multicellular system, several vital parts must be taken into consideration. We use a toggle switch to save a status, which simulates the cell differentiation, a AHL generate-receive system for intercelluar communication, an antisense mRNA biobrick as cell division inhibitor, in order to control the shape of bacteria colony. We designed a cell movement control device to start the differentiation automatically, as well as a light sensor system to start it artificially for easier control and further application.

Light Sensor

Toggle Switch

Intercelluar Communication

Cell Division Inhibitor


Retrieved from "http://2012.igem.org/Team:SEU_O_China/Project"