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| | <font color="#232323"> Human Practise </font></a></li> | | <font color="#232323"> Human Practise </font></a></li> |
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| - | p> </p>
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| - | <h2 style="font-variant: normal; vertical-align: baseline; clear: left; color: #232323; font-family: Gentium Basic; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-align: left; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin: 0.7em 0px; padding: 0px">
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| - | <span style="font-weight: 400; text-decoration:underline">
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| - | <font face="Trebuchet MS" size="6">Abstract</font></span></h2>
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| - | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: rgb(85, 85, 85); font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">
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| - | </p>
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| - | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">
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| - | HKU’s iGEM team aims to introduce
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| - | an acyl homoerine lactone (AHL)-degrading genetic system into the non-biolfilm-forming
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| - | and non-virulent BL21 Escherichia coli strain. PvdQ, an enzyme naturally
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| - | produced by Pseudomonas aeruginosa, is an acylase that functions to
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| - | degrade long chain AHLs that bacteria like Pseudomonas putida or
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| - | aeruginosa itself utilize for biofilm formation. Biofilms are population
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| - | density-dependent structures formed by quorum sensing bacteria that
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| - | produce and secrete auto-inducers, which signal selective gene
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| - | transcription. These signaling molecules, namely the AHLs, are
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| - | responsible for most bacterial pathogenicity including the opportunistic
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| - | respiratory infections caused by P.aeuroginosa in immunocompromised
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| - | patients. </p>
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| - | <p style="text-align: left; font-style: normal; font-variant: normal; font-weight: normal; font-size: 13px; vertical-align: baseline; color: #232323; font-family: Lato, Tahoma, Arial, sans-serif; letter-spacing: normal; line-height: 19.5px; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; border: 0px none; margin-left: 0px; margin-right: 0px; margin-top: 0px; margin-bottom: 20px; padding: 0px">
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| - | As a step towards combating these infections, E.coli can be effectively
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| - | used as a protein factory to maximize pvdQ yield in vitro or ex vivo.
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| - | Our most preliminary biobrick is a constitutive promoter that drives
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| - | baseline, exponential expression of pvdQ. This genetic pathway is
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| - | advantageous because the pvdQ gene is constitutively transcribed
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| - | regardless of environmental and endogenous factors. </p>
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| - | <br></div>
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| | <p style="text-align:center;"> | | <p style="text-align:center;"> |
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