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Team:Macquarie Australia/Protocols/ligations
From 2012.igem.org
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<center><h1>Ligation Procedure</h1></center> | <center><h1>Ligation Procedure</h1></center> | ||
<p>A reaction mixture was prepared containing,<br> | <p>A reaction mixture was prepared containing,<br> | ||
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<td>11 µL</td></tr> | <td>11 µL</td></tr> | ||
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Revision as of 00:24, 21 September 2012
Ligation Procedure
A reaction mixture was prepared containing,
| Element | Volume |
| Upstream Digestion part | 2 µL |
| Downstream Digestion part | 2 µL |
| Destination Plasmid | 2 µL |
| 10X T4 DNA ligase buffer | 2 µL |
| T4 DNA ligase | 1 µL |
| H2O | 11 µL |
