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Team:Macquarie Australia/Protocols/HO
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Revision as of 09:39, 20 September 2012
Heme Oxygenase Characterisation
Rationale
To determine if our heme oxygenase BioBrick part was functional we needed to stimulate the pathway. We could easily determined if heme oxygenase was functional due to the production of the pigment biliverdine. As our BioBrick was under control of the T7 promoter, the enzyme would need to be induced using IPTG. The heme pathway was also stimulated using d-aminolevulinic acid (ALA). If our Heme oxygenase was functional than the cells would be green in appearance.
Protocol
- After transforming BL21 E. coli
