Team:Austin Texas/Plasmids and Strains

From 2012.igem.org

(Difference between revisions)
(→ZombiE.coli parts)
(→Plasmid Database)
Line 6: Line 6:
! Box
! Box
! Slot
! Slot
 +
! Label
! Name
! Name
-
! Description
+
! Part
 +
! Plasmid
! Source
! Source
|-
|-
| iGEM 2012 Plasmid Box 1
| iGEM 2012 Plasmid Box 1
| 2
| 2
 +
|
| cadA
| cadA
 +
|
| ASKA cadA plasmid
| ASKA cadA plasmid
| Barrick Lab
| Barrick Lab
Line 18: Line 22:
| iGEM 2012 Plasmid Box 1
| iGEM 2012 Plasmid Box 1
| 3
| 3
 +
|
| ldcC
| ldcC
 +
|
| ASKA ldcC plasmid
| ASKA ldcC plasmid
| Barrick Lab
| Barrick Lab
|-
|-
 +
| iGEM 2012 Plasmid Box 1
 +
|
 +
| IGM004
 +
| GFP
 +
| [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891]
 +
| pSB3K3
 +
| 2012 kit plate 2 (15L)
 +
|-
 +
| iGEM 2012 Plasmid Box 1
 +
|
 +
| IGM005
 +
| RFP
 +
| [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014]
 +
| pSB1AK3
 +
| 2012 kit plate 3 (12O)
 +
|-
 +
| iGEM 2012 Plasmid Box 1
 +
|
 +
| IGM006
 +
| LasI
 +
| [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009]
 +
| pSB1A2
 +
| 2012 kit plate 2 (10N)
|}
|}

Revision as of 18:26, 14 June 2012

Plasmid Database

All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.

Box Slot Label Name Part Plasmid Source
iGEM 2012 Plasmid Box 1 2 cadA ASKA cadA plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 3 ldcC ASKA ldcC plasmid Barrick Lab
iGEM 2012 Plasmid Box 1 IGM004 GFP [http://www.partsregistry.org/Part:BBa_I714891 BBa_I714891] pSB3K3 2012 kit plate 2 (15L)
iGEM 2012 Plasmid Box 1 IGM005 RFP [http://www.partsregistry.org/Part:BBa_K081014 BBa_K081014] pSB1AK3 2012 kit plate 3 (12O)
iGEM 2012 Plasmid Box 1 IGM006 LasI [http://www.partsregistry.org/Part:BBa_K081009 BBa_K081009] pSB1A2 2012 kit plate 2 (10N)

Strain Database

All strains are in the back right rack of the –80°C freezer.

Box Slot Name Description Source
iGEM 2012 Box 1 2 AG1 Host strain of ASKA collection Barrick Lab
iGEM 2012 Box 1 3 BW25113 Host strain of Keio collection Barrick Lab
iGEM 2012 Box 1 4 cadA AG1 + ASKA collection cadA plasmid, CamR Barrick Lab
iGEM 2012 Box 1 5 ldcC AG1 + ASKA collection ldcC plasmid, CamR Barrick Lab
iGEM 2012 Box 1 6 tnaA Keio collection knockout (version of odor-less E. coli), KanR Barrick Lab

ZombiE.coli parts

Plasmid Part Description Source
pReporter [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) 2012 kit plate 2 (15L)
pReporter [http://partsregistry.org/Part:BBa_K081014 BBa_K081014] RFP protein generator - PoPS->RFP 2012 kit plate 3 (12O)
pReporter [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] LasI protein generator (TERM-) 2012 kit plate 2 (10N)
pReporter [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] RBS + LacI 2012 kit plate 1 (7L)
pReporter [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] Double terminator (BBa_B0010-BBa_B0012) 2012 kit plate 1 (23L)
pConverter [http://partsregistry.org/Part:BBa_R0079 BBa_R0079] LasR Promoter 2012 kit plate 1 (12A)
pConverter [http://partsregistry.org/Part:BBa_C0079 BBa_C0079] LasR 2012 kit plate 1 (14J)
pConverter [http://partsregistry.org/Part:BBa_K082016 BBa_K082016] LacIM1 Parts Registry
pConverter [http://partsregistry.org/Part:BBa_R0010 BBa_R0010] LacI Promoter 2012 kit plate 1 (1D)
pConverter CRE pQL269 Plasmid
Retrieved from "http://2012.igem.org/Team:Austin_Texas/Plasmids_and_Strains"