Team:LMU-Munich/Test

From 2012.igem.org

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{| style="color:black;" cellpadding="10" width="70%" cellspacing="0" border="0" align="center" style="text-align:center;"
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
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| style="width: 70%;background-color:#116f3d;" |
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
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{|
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
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|[[File:Imamura, 2011 &amp; McKenney, 2010.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px]]
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| style="width:20%"|'''[[Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags|Affinity tags]]
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| style="width: 70%;background-color:#116f3d;" |<font color="#EBFCE4">Protein distribution in spore coat of ''Bacillus subtilis''</font>
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
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|[[File:LMU PromoterIconBC.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters]]
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|[[File:LMU Reporter.png|60px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters]]
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|[[File:Proteinaffinitytagbutton.png|50px|link=Team:LMU-Munich/Bacillus_BioBricks#Affinity_Tags]]
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We will create a toolbox of <i>Bacillus</i> BioBricks to contribute to the registry.
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====Project Navigation====
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This ''<b>Bacillus''B</b>io<b>B</b>rick<b>B</b>ox contains ''Bacillus'' specific:
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|[[File:Bacilluss_Intro.png|100px|link=Team:LMU-Munich/Bacillus_Introduction]]
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|[[File:BacillusBioBrickBox.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks]]
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|[[File:SporeCoat.png|100px|link=Team:LMU-Munich/Spore_Coat_Proteins]]
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|[[File:GerminationSTOP.png|100px|link=Team:LMU-Munich/Germination_Stop]]
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{| style="background-color:white" cellpadding="0" width="100%" cellspacing="0" border="0" align="center" style="text-align:center;"
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| style="width:20%"|
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]'''
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| style="width:20%"|
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Promoters|Promoters]]'''
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| style="width:20%"|
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Reporters|Reporters]]'''
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| style="width:20%"|
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'''[[Team:LMU-Munich/Bacillus_BioBricks#Tags|Affinity tags]]
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|-
|-
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|[[File:LMU Backbone.png|100px|link=Team:LMU-Munich/Bacillus_BioBricks#Bacillus_Vectors|Vectors]]
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|[[Team:LMU-Munich/Bacillus_Introduction|<font size="1">Bacillus<BR>Intro</font>]]
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|[[File:LMU PromoterIconBC.png|100px]]
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|[[Team:LMU-Munich/Bacillus_BioBricks|<font size="1" face="verdana">Bacillus<BR>BioBrickBOX</font>
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|[[File:LMU Reporter.png|60px]]
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|[[Team:LMU-Munich/Spore_Coat_Proteins|<font size="1" face="verdana">SporeCoat<BR>FusionProteins</font>
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|[[File:Proteinaffinitytagbutton.png|50px]]
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|[[Team:LMU-Munich/Germination_Stop|<font size="1" face="verdana">Germination<BR>STOP</font>
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</div>
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{| class="colored"
 
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!Name BioBrick
 
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!''E. coli'' res.
 
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!''B. subt.'' res.
 
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!Insertion locus
 
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!Description
 
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!Derived from
 
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!Reference
 
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|-
 
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!pSB<sub>Bs</sub>1C-<i>lacZ</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 <font color="#EBFCE4" size="2">(BBa_K823021) </font>]
 
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|Amp
 
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|Cam
 
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|amyE
 
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|with <i>lacZ</i> reporter gene
 
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|pAC6
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke ''et al''.]
 
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|-
 
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!pSB<sub>Bs</sub>4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 <font color="#EBFCE4" size="2">(BBa_K823022)</font>]
 
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|Amp
 
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|Spec
 
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|thrC
 
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|empty
 
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|pDG1731
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
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|-
 
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!pSB<sub>Bs</sub>1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 <font color="#EBFCE4" size="2">(BBa_K823023)</font>]
 
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|Amp
 
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|Cam
 
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|amyE
 
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|empty
 
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|pDG1662
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury ''et al.'']
 
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|-
 
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!pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 <font color="#EBFCE4" size="2">(BBa_K823024)</font>]
 
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|Amp
 
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|Spec
 
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|thrC
 
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|with Xylose-inducible promoter
 
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|pXT
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré ''et al.'']
 
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|-
 
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!pSB<sub>Bs</sub>3C-<i>luxABCDE</i> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 <font color="#EBFCE4" size="2">(BBa_K823025)</font>]
 
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|Amp
 
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|Cam
 
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|sacA
 
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|with <i>luxABCDE</i> reporter cassette
 
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|pAH328
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch ''et al''.]
 
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|-
 
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!pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 <font color="#EBFCE4" size="2">(BBa_K823026)</font>]
 
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|Amp
 
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|Kan
 
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|replicative
 
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|with IPTG inducible promoter
 
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|pDG148
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph ''et al.'']
 
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|-
 
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!pSB<sub>Bs</sub>2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 <font color="#EBFCE4" size="2">(BBa_K823027)</font>]
 
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|Amp
 
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|MLS
 
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|lacA
 
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|empty
 
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|pAX01
 
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|[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.'']
 
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|-
 
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Revision as of 19:22, 19 September 2012

iGEM Ludwig-Maximilians-Universität München Beadzillus

Team-LMU Photo1.jpg

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

IGEM HQ LMU prize.jpg

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