Team:LMU-Munich/Test

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Revision as of 15:54, 19 September 2012

The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".

[ more news ]

The promoters we submit are:

Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR

We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in Bacillus subtilis with pSB_Bs3C-luxABCDE. The data will be online in our Data section soon.

Furthermore we plan to submit reporter genes optimized for Bacillus subtilis, namely GFP, lacZ, luc and mKate2.

Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).

More details to come soon.

Vectors	Promoters	Reporters	Affinity tags

pSB_Bs1C pSB_Bs4S pSB_Bs2E pSB_Bs1C-lacZ pSB_Bs3C-luxABCDE pSB_Bs4S-P_Xyl pSB_Bs0K-P_spac	Pveg PliaI PlepA Anderson	mkate2 luc lacZ	Flag His cMyc Strep HA

BacillusBioBrickBox

Anderson Promoter Evaluation

Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Anderson Promoter Evaluation


Evaluation of the Anderson library in B. subtilis with pSB_Bs3C-lux (luminescence) and pSB_Bs1C-lacZ

Protein distribution in spore coat of Bacillus subtilis

We will create a toolbox of Bacillus BioBricks to contribute to the registry.

This BacillusBioBrickBox contains Bacillus specific:

Vectors	Promoters	Reporters	Affinity tags

Name BioBrick	E. coli res.	B. subt. res.	Insertion locus	Description	Derived from	Reference
pSB_Bs1C-lacZ [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823021 (BBa_K823021) ]	Amp	Cam	amyE	with lacZ reporter gene	pAC6	[http://www.ncbi.nlm.nih.gov/pubmed/11902727 Stülke et al.]
pSB_Bs4S [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823022 (BBa_K823022)]	Amp	Spec	thrC	empty	pDG1731	[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]
pSB_Bs1C [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823023 (BBa_K823023)]	Amp	Cam	amyE	empty	pDG1662	[http://www.ncbi.nlm.nih.gov/pubmed/8973347 Guérout-Fleury et al.]
pSB_Bs4S-P_Xyl [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823024 (BBa_K823024)]	Amp	Spec	thrC	with Xylose-inducible promoter	pXT	[http://www.ncbi.nlm.nih.gov/pubmed/11069659 Derré et al.]
pSB_Bs3C-luxABCDE [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823025 (BBa_K823025)]	Amp	Cam	sacA	with luxABCDE reporter cassette	pAH328	[http://www.ncbi.nlm.nih.gov/pubmed/20709900 Schmalisch et al.]
pSB_Bs0K-P_spac [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823026 (BBa_K823026)]	Amp	Kan	replicative	with IPTG inducible promoter	pDG148	[http://www.ncbi.nlm.nih.gov/pubmed/11728721 Joseph et al.]
pSB_Bs2E [http://partsregistry.org/wiki/index.php?title=Part:BBa_K823027 (BBa_K823027)]	Amp	MLS	lacA	empty	pAX01	[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]

Retrieved from "http://2012.igem.org/Team:LMU-Munich/Test"

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+The promoters we submit are:
+Pveg, PliaI, PliaG, plepA, Pxyl, Pxyl+XylR
+<p align="justify">We also tested the [http://partsregistry.org/Promoters/Catalog/Anderson Anderson promoter collection] in ''Bacillus subtilis'' with pSB<sub>Bs</sub>3C-<i>luxABCDE</i>. The data will be online in our [https://2012.igem.org/Team:LMU-Munich/Data Data] section soon.</p>
+<p align="justify">Furthermore we plan to submit reporter genes optimized for ''Bacillus subtilis'', namely ''GFP'', ''lacZ'', ''luc'' and ''mKate2''. </p>
+Useful for the registry are also 5 tags in Freiburg Standard (cMyc, 10His, Flag, Strep, HA).
+More details to come soon.
 <div class="box">