Team:WashU/Protocols/PCR

From 2012.igem.org

(Difference between revisions)
Xu740 (Talk | contribs)
(Created page with "=General PCR Protocol= #Setup Amplification PCR Reaction ##1uL Template ##1uL 25mM dNTP's ##10uL Phusion HF Buffer ##0.5uL Forward Primer (Tm XX <sup>o</sup>C)* ##0.5uL Reverse ...")
Newer edit →

Revision as of 20:21, 13 June 2012

General PCR Protocol

  1. Setup Amplification PCR Reaction
    1. 1uL Template
    2. 1uL 25mM dNTP's
    3. 10uL Phusion HF Buffer
    4. 0.5uL Forward Primer (Tm XX oC)*
    5. 0.5uL Reverse Primer (Tm YY oC)*
    6. 0.5uL Phusion polymerase
    7. 36.5uL diH2O
Retrieved from "http://2012.igem.org/Team:WashU/Protocols/PCR"