Team:Cambridge/Protocols/TAEBuffer

From 2012.igem.org

(Difference between revisions)
(10xTAE buffer)
(10xTAE buffer)
Line 7: Line 7:
11.4ml acetic acid
11.4ml acetic acid
-
3.7g EDTA
+
3.7g EDTA (35ml of 0.5M EDTA)
*Make up to 900ml with DI water
*Make up to 900ml with DI water

Revision as of 16:33, 18 September 2012

10xTAE buffer

48.4g TRIS

11.4ml acetic acid

3.7g EDTA (35ml of 0.5M EDTA)

  • Make up to 900ml with DI water
  • Adjust with NaOH to pH = 8.0
  • fill to 1L