Team:Goettingen/week8-3

From 2012.igem.org

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<h2><b>V06_21 </b></h2><br>
<h2><b>V06_21 </b></h2><br>
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<b>Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
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<b>V06_21_1 Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
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<ul>
<li>Experiment: <br>All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol.</a></li>
<li>Experiment: <br>All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol.</a></li>
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</ul>
</ul>
<br>
<br>
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<b>Preparative digest of promoter_TAR_QC constructs and pSB1C3</b><br>
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<b>V06_21_2 Preparation of glycerol stocks <i>E. coli</i> DH10B with pSB1C3</b><br>
<ul>
<ul>
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<li>Experiment: <br>All 8 promoter constructs + TAR_QC in pUC18 and the BioBrick vector pSB1C3 were digested according to <a href="https://2012.igem.org/Team:Goettingen/Project/Methods">protocol.</a></li>
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<li>Experiment: <br>Glycerol stocks (30 %) were of 2 clones were prepared and stored at -80 °C.</a></li>
</ul>
</ul>
<ul>
<ul>
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<li>Observations and results: <br>The transformation of DH10B with pSB1C3 (see V06_18) worked well.
 
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Revision as of 11:32, 17 September 2012

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