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Team:Wageningen UR/Journal/week17
From 2012.igem.org
Lisamsonne (Talk | contribs) (→week 17: 20 august - 26 august) |
Lisamsonne (Talk | contribs) (→week 17: 20 august - 26 august) |
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* Transformation | * Transformation | ||
| - | Both into JM109 and DH5α; using the BBa_J04450 brick as positive control | + | - Both into JM109 and DH5α; using the BBa_J04450 brick as positive control |
| + | |||
| + | |||
| + | ''22 August'' | ||
| + | * Colony PCR | ||
| + | - Of the transformations HepB + IPTGpromoter in BBa_J04450 backbone in JM109 and HepB + IPTGpromoter in BBa_J04450 backbone in DH5α | ||
| + | |||
'''Hepatitis B inside modification''' | '''Hepatitis B inside modification''' | ||
---- | ---- | ||
| + | ''23 August'' | ||
| + | * Step 3 and step 4 of the PCR reaction | ||
| + | -> no bands visible for the 3rd and 4th step of HepBinside-coil | ||
| - | + | * control of a miniprep of HepB+IPTG in BL21 from 16.Aug (sample from 6.Aug) | |
| + | -> the miniprep shows a band of the expected size - so the transformation from 6.Aug was successful | ||
'''GFP modification''' | '''GFP modification''' | ||
| Line 36: | Line 46: | ||
* Transformation | * Transformation | ||
Both into JM109 and DH5α; using the BBa_J04450 brick as positive control | Both into JM109 and DH5α; using the BBa_J04450 brick as positive control | ||
| + | |||
| + | |||
| + | ''22 August'' | ||
| + | |||
| + | * Colony PCR | ||
| + | Of the transformations GFP-coil in BBa_J04450 backbone in JM109 and GFP-coil in BBa_J04450 backbone in DH5α | ||
Revision as of 09:58, 17 September 2012
week 17: 20 august - 26 august
Office work
Lab work
Hepatitis B
20 August
- Colony PCR
- of HepB (without promoter) in BBa_J04450 - of HepB with a his tag + IPTG promoter in Bba_J04500
- Ligation
- of the HepB with the IPTG promoter into BBa_J04450
- Transformation
- Both into JM109 and DH5α; using the BBa_J04450 brick as positive control
22 August
- Colony PCR
- Of the transformations HepB + IPTGpromoter in BBa_J04450 backbone in JM109 and HepB + IPTGpromoter in BBa_J04450 backbone in DH5α
Hepatitis B inside modification
23 August
- Step 3 and step 4 of the PCR reaction
-> no bands visible for the 3rd and 4th step of HepBinside-coil
- control of a miniprep of HepB+IPTG in BL21 from 16.Aug (sample from 6.Aug)
-> the miniprep shows a band of the expected size - so the transformation from 6.Aug was successful
GFP modification
20 August
- Ligation
- of the digested GFP-coil PCR product from 15.Aug into BBa_J04450
- Transformation
Both into JM109 and DH5α; using the BBa_J04450 brick as positive control
22 August
- Colony PCR
Of the transformations GFP-coil in BBa_J04450 backbone in JM109 and GFP-coil in BBa_J04450 backbone in DH5α
