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Team:Carnegie Mellon/Met-Protocols
From 2012.igem.org
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<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| - | <p><h2 id = "section1 | + | <p><h2 id = "section1"> |
<b>Overview</b></h2> | <b>Overview</b></h2> | ||
| + | <id = "section1-1"> | ||
Our protocols are based on standard lab protocols used in the lab of our advisers unless otherwise stated. These protocols were subsequently modified if necessary based on our experience. A knowledge of basic lab techniques such as pippeting, plating, etc. are assumed and not covered as there are pretty comprehensive tutorials on the web for those. | Our protocols are based on standard lab protocols used in the lab of our advisers unless otherwise stated. These protocols were subsequently modified if necessary based on our experience. A knowledge of basic lab techniques such as pippeting, plating, etc. are assumed and not covered as there are pretty comprehensive tutorials on the web for those. | ||
</p> | </p> | ||
| - | <p><h2 | + | <p><h2> |
<b>Dosage Curve</b></h2> | <b>Dosage Curve</b></h2> | ||
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<table> | <table> | ||
<tr> <td width = "25"> 1. </td><td width = "100">Wash 1mL induced cells once with PBS and resuspend in 1mL M9 media.</td></tr> | <tr> <td width = "25"> 1. </td><td width = "100">Wash 1mL induced cells once with PBS and resuspend in 1mL M9 media.</td></tr> | ||
Revision as of 03:08, 17 September 2012
Protocols
Overview
Dosage Curve
| 1. | Wash 1mL induced cells once with PBS and resuspend in 1mL M9 media. |
| 2. | Add 1µL of 50nM Mg2+ as MgCl2 from PCR kit to the tubes |
