"
Team:Carnegie Mellon/Met-Protocols
From 2012.igem.org
(Difference between revisions)
| Line 101: | Line 101: | ||
<a href="#" class="toc-link" id="toc-link"><span>▼</span> Table of Contents</a> | <a href="#" class="toc-link" id="toc-link"><span>▼</span> Table of Contents</a> | ||
<ul id="toc" class="toc-new" style="background: #c1a562;"> | <ul id="toc" class="toc-new" style="background: #c1a562;"> | ||
| - | <li class="toc-h1"><a href="#section1"> | + | <li class="toc-h1"><a href="#section1">Introduction</a> |
<ul class="toc-sub closed"> | <ul class="toc-sub closed"> | ||
| - | <li><a href="#section1-1"> | + | <li><a href="#section1-1">Overview</a></li> |
| - | + | ||
| - | + | ||
</ul> | </ul> | ||
</li> | </li> | ||
| Line 115: | Line 113: | ||
<h1>Protocols</h1> | <h1>Protocols</h1> | ||
| - | <p><h2 id = "section1"> | + | <p><h2 id = "section1-1"> |
<b>Overview</b></h2> | <b>Overview</b></h2> | ||
Our protocols are based on standard lab protocols used in the lab of our advisers unless otherwise stated. These protocols were subsequently modified if necessary based on our experience. A knowledge of basic lab techniques such as pippeting, plating, etc. are assumed and not covered as there are pretty comprehensive tutorials on the web for those. | Our protocols are based on standard lab protocols used in the lab of our advisers unless otherwise stated. These protocols were subsequently modified if necessary based on our experience. A knowledge of basic lab techniques such as pippeting, plating, etc. are assumed and not covered as there are pretty comprehensive tutorials on the web for those. | ||
| + | </p> | ||
| + | |||
| + | <p><h2 id = "section2-1"> | ||
| + | <b>Dosage Curve</b></h2> | ||
| + | <table> | ||
| + | <tr> <td width = "25"> 1. </td><td width = "100">Wash 1mL induced cells once with PBS and resuspend in 1mL M9 media.</td></tr> | ||
| + | <tr><td>2. </td><td>Add 1µL of 50nM Mg2+ as MgCl2 from PCR kit to the tubes</td></tr> | ||
| + | </table> | ||
| + | |||
</p> | </p> | ||
Revision as of 03:06, 17 September 2012
Protocols
Overview
Our protocols are based on standard lab protocols used in the lab of our advisers unless otherwise stated. These protocols were subsequently modified if necessary based on our experience. A knowledge of basic lab techniques such as pippeting, plating, etc. are assumed and not covered as there are pretty comprehensive tutorials on the web for those.Dosage Curve
| 1. | Wash 1mL induced cells once with PBS and resuspend in 1mL M9 media. |
| 2. | Add 1µL of 50nM Mg2+ as MgCl2 from PCR kit to the tubes |
