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Team:Austin Texas/Plasmids and Strains
From 2012.igem.org
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| [[File:BBa_J24679.fasta.txt]] | | [[File:BBa_J24679.fasta.txt]] | ||
| 2012 kit plate 1 (7L) | | 2012 kit plate 1 (7L) | ||
| + | |- | ||
| + | | pReporter | ||
| + | | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | ||
| + | | Double terminator (BBa_B0010-BBa_B0012) | ||
| + | | [[File:BBa_B0015.fasta.txt]] | ||
| + | | 2012 kit plate 1 (23L) | ||
|} | |} | ||
Revision as of 19:59, 12 June 2012
Plasmid Database
All plasmid stocks are in the –20°C freezer. Slots are labelled 1 through 81 in each box.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Plasmid Box 1 | 2 | cadA | ASKA cadA plasmid | Barrick Lab |
| iGEM 2012 Plasmid Box 1 | 3 | ldcC | ASKA ldcC plasmid | Barrick Lab |
Strain Database
All strains are in the back right rack of the –80°C freezer.
| Box | Slot | Name | Description | Source |
|---|---|---|---|---|
| iGEM 2012 Box 1 | 2 | AG1 | Host strain of ASKA collection | Barrick Lab |
| iGEM 2012 Box 1 | 3 | BW25113 | Host strain of Keio collection | Barrick Lab |
| iGEM 2012 Box 1 | 4 | cadA | AG1 + ASKA collection cadA plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 5 | ldcC | AG1 + ASKA collection ldcC plasmid, CamR | Barrick Lab |
| iGEM 2012 Box 1 | 6 | tnaA | Keio collection knockout (version of odor-less E. coli), KanR | Barrick Lab |
ZombiE.coli parts
| Plasmid | Part | Description | Sequence | Source |
|---|---|---|---|---|
| pReporter | [http://partsregistry.org/Part:BBa_I714891 BBa_I714891] | GFP: Green fluorescent protein (fused with SDY... is there a different part we should use?) | File:BBa I714891.fasta.txt | 2012 kit plate 2 (15L) |
| pReporter | [http://partsregistry.org/Part:BBa_J06504 BBa_J06504] | Monomeric RFP optimized for bacteria | File:BBa J06504.fasta.txt | 2012 kit plate 1 (13F) |
| pReporter | [http://partsregistry.org/Part:BBa_K081009 BBa_K081009] | LasI protein generator (TERM-) | File:BBa S03967.fasta.txt | 2012 kit plate 2 (10N) |
| pReporter | [http://partsregistry.org/Part:BBa_J24679 BBa_J24679] | RBS + LacI | File:BBa J24679.fasta.txt | 2012 kit plate 1 (7L) |
| pReporter | [http://partsregistry.org/Part:BBa_B0015 BBa_B0015] | Double terminator (BBa_B0010-BBa_B0012) | File:BBa B0015.fasta.txt | 2012 kit plate 1 (23L) |
