Team:Carnegie Mellon/Bio-Submitted

From 2012.igem.org

(Difference between revisions)
(Created page with "{{:Team:Carnegie_Mellon/Templates/Header}} <html> <!-- Nav Bar --> <ul class="sf-menu sf-navbar"> <li style ='width: 193px'> <a href="https://2012.igem.org/Team:Carneg...")
Line 101: Line 101:
<a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a>
<a href="#" class="toc-link" id="toc-link"><span>&#9660;</span> Table of Contents</a>
<ul id="toc" class="toc" style="background: #ac9d74;">
<ul id="toc" class="toc" style="background: #ac9d74;">
-
<li class="toc-h1"><a href="#section1">1. FAQ</a>
+
<li class="toc-h1"><a href="#section1">Submitted Parts</a>
<ul class="toc-sub closed">
<ul class="toc-sub closed">
-
<li><a href="#section1-1">1.1 Question 1</a></li>
+
<li><a href="#pre-experiment">Hypothesis</a></li>
-
<li><a href="#section1-2">1.2 Question 2</a></li>
+
<li><a href="#promoter">Promoter Strength</a></li>
-
<li><a href="#section1-3">1.2 Question 3</a></li>
+
<li><a href="#leaky">Leaky Expression</a></li>
</ul>
</ul>
</li>
</li>
Line 113: Line 113:
   <div class = "main_content">
   <div class = "main_content">
 +
<h1 class="section1"> Submitted Parts </h1>
 +
<p>
 +
BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA <br>
 +
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA <br>
 +
Mutant II: CAATACGACTCACTATAGGGCAATTGTGAGCGCTCACAATTGCAA <br>
 +
Mutant III: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA <br>
 +
Mutant IV: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA <br>
 +
</p><p class="promoter">
 +
<h3 class="pre-experiment">Hypothesis:</h3>
 +
Expected promoter strength of different mutants:<br>
 +
Mutant I: <100% <br>
 +
Mutant II: >>100% <br>
 +
Mutant III: ~100% <br>
 +
Mutant IV: ~50%<br>
 +
</p><p class="leaky">
 +
Expected LacI leaky expression of different mutants: <br>
 +
Mutant I: More than average <br>
 +
Mutant II: Below average <br>
 +
Mutant III: Average <br>
 +
Mutant IV: Average <br>
 +
</p>
</html>
</html>
{{:Team:Carnegie_Mellon/Templates/Footer}}
{{:Team:Carnegie_Mellon/Templates/Footer}}

Revision as of 21:14, 15 September 2012

Image:CMU_image6.jpeg




Submitted Parts

BBa_K613007: TAATACGACTCACTATAGGGAGAGGAATTGTGAGCGGATAACAA
Mutant I: TAATGCGACTCACTATAGGACAATTGTGGGCGGACAACAATTCCAA
Mutant II: CAATACGACTCACTATAGGGCAATTGTGAGCGCTCACAATTGCAA
Mutant III: TAATACGACTCACTACAGGGCGGAATTGTGAGCGGATAACAATTCCAA
Mutant IV: CAATCCGACTCACTAAAGAGAGAATTGTGAGCGGATAACAATTCCAA

Hypothesis:

Expected promoter strength of different mutants:
Mutant I: <100%
Mutant II: >>100%
Mutant III: ~100%
Mutant IV: ~50%

Expected LacI leaky expression of different mutants:
Mutant I: More than average
Mutant II: Below average
Mutant III: Average
Mutant IV: Average

Image:TartanFooter.jpeg