From 2012.igem.org
(Difference between revisions)
|
|
| Line 233: |
Line 233: |
| | </ul> | | </ul> |
| | | | |
| - | Update for June 25
| + | <p><b>June 25</b></p> |
| | | | |
| - | Wet Lab | + | <p>Wet Lab</p> |
| - | Ashwin/Avin - Column purification, ligation, and transformation of Pet26b-mCherry | + | <ul> |
| - | Mike -
| + | <li>Ashwin/Avin - Column purification, ligation, and transformation of Pet26b-mCherry</li> |
| - | Peter -run gel of eGFP/plsr ligation | + | <li>Peter -run gel of eGFP/plsr ligation</li> |
| - | Nikita -
| + | </ul> |
| | | | |
| - | Dry Lab | + | <p>Dry Lab</p> |
| - | Avin - Sent in final gene synthesis order | + | <ul> |
| - | Mike - reviewed pDawn protocol, reviewed TetR sequences | + | <li>Avin - Sent in final gene synthesis order</li> |
| - | Peter- Order restriction enzymes from cell center | + | <li>Mike - reviewed pDawn protocol, reviewed TetR sequences</li> |
| | + | <li>Peter- Order restriction enzymes from cell center</li> |
| | | | |
| | | | |
Revision as of 10:48, 15 September 2012
Penn 2012 iGEM Wiki
June 2012 Notebook
June 6th
- Set up some lab equipment
- Autoclaved for a while
- Organized biobrick stuff
- Called Vinoo about DNA planning
June 7th
- Transformed Cph8, pLsr, and LuxS
- Placed order with Vinoo
- Developed idea using PGY/PCN system to activate a gene
June 11th
Wet Lab
- PCR'd mCherry from NAS157
- Ran 1% Gel and purified product
Dry Lab
- Designed primers for LsR promoter
- Meeting with Dr. Sarkar
June 12th
Wet Lab
- Digested mCherry PCR product with BamHI and NotI
- Column purified mCherry and ligated into NAS152 backbone
- Transformed NAS152-mCherry into DH5alpha
- Poured 25 LB-Kan plates
Dry Lab
- Research more information about bacterial drug delivery system
- More research into biofilm project
June 14th
Wet Lab
Dry Lab
- Met with Dr. Goulian, obtained pDawn and pDusk
- Identified inaK as a surface display gene we can use
June 18th
Wet Lab
- Miniprep pDawn and pDusk
- Test cut pDawn and pDusk with XmaI, analytical gel was correct
- Prep cut pDawn and pDusk with BamHI and NotI, gel purified
Dry Lab
- Ordered and picked up PCR purification kit from cell center
- Additional orders through cell center
- Designed primers for one of Peter's components (forgot which)
June 20
Wet Lab
- Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
- PCR purified fragments (Peter), then ran gel?
Dry Lab
- Researched DARPin binding domains and linkers
- Finalized some biobrick orders
- Finalized synthesis order (minus linker)
June 22
Wet Lab
- Ashwin - Repeated miniprep on pDawn and did test cut
- Peter - Miniprepped pet26b and digested with BglII and EcorRI
- Avin - Miniprepped pet26b, made glycerol stock and digested with BamH1 and Not1
Dry Lab
- Avin - Finalized and sent in synthesis order (still awaiting order confirmation)
June 25
Wet Lab
- Ashwin/Avin - Column purification, ligation, and transformation of Pet26b-mCherry
- Peter -run gel of eGFP/plsr ligation
Dry Lab
- Avin - Sent in final gene synthesis order
- Mike - reviewed pDawn protocol, reviewed TetR sequences
- Peter- Order restriction enzymes from cell center
July 2012 Notebook
"
