Team:Penn/Notebook

From 2012.igem.org

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<h2 class="accordion-header">Week 1</h2>
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<div class="accordion-content">
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<p><b>June 6th</b></p>
 +
                                                <ul>
 +
                                                  <li>Set up some lab equipment</li>
 +
                                                  <li>Autoclaved for a while</li>
 +
<li>Organized biobrick stuff</li>
 +
                                                  <li>Called Vinoo about DNA planning</li>
 +
 
 +
                                                </ul> 
 +
<br>
 +
 +
<p><b>June 7th</b></p>
 +
                                                <ul>
 +
                                                  <li>Transformed Cph8, pLsr, and LuxS</li>
 +
                                                  <li>Placed order with Vinoo</li>
 +
<li>Developed idea using PGY/PCN system to activate a gene</li>
 +
                                                </ul>                   
 +
 +
 +
</div>
</div>
 +
 +
<h2 class="accordion-header">Week 2</h2>
 +
 +
<div class="accordion-content">
 +
 +
<p><b>June 11th</b></p>
 +
                            &nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>PCR'd mCherry from NAS157</li>
 +
                                                  <li>Ran 1% Gel and purified product</li>
 +
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Designed primers for LsR promoter</li>
 +
<li>Meeting with Dr. Sarkar</li>
 +
</ul>
 +
<br>
 +
<p><b>June 12th</b></p>
 +
                            &nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>Digested mCherry PCR product with BamHI and NotI</li>
 +
                                                  <li>Column purified mCherry and ligated into NAS152 backbone</li>
 +
<li>Transformed NAS152-mCherry into DH5alpha</li>
 +
<li>Poured 25 LB-Kan plates</li>
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Research more information about bacterial drug delivery system</li>
 +
<li>More research into biofilm project</li>
 +
</ul>
 +
<br>
 +
<p><b>June 14th</b></p>
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>Fill in later....</li>
 +
                                               
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Met with Dr. Goulian, obtained pDawn and pDusk</li>
 +
<li>Identified inaK as a surface display gene we can use</li>
 +
</ul>
 +
</div>
 +
 +
<h2 class="accordion-header">Week 3</h2>
 +
 +
<div class="accordion-content">
 +
 +
<p>June 18th</p>
 +
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
<ul>
 +
<li>Miniprep pDawn and pDusk</li>
 +
<li>Test cut pDawn and pDusk with XmaI, analytical gel was correct</li>
 +
<li>Prep cut pDawn and pDusk with BamHI and NotI, gel purified</li>
 +
</ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Ordered and picked up PCR purification kit from cell center</li>
 +
<li>Additional orders through cell center</li>
 +
<li>Designed primers for one of Peter's components (forgot which)</li>
 +
</ul>
 +
<br>
 +
 +
<p>June 20</p>
 +
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
<ul>
 +
<li>Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan</li>
 +
<li>PCR purified fragments (Peter), then ran gel?</li>
 +
</ul>
 +
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Researched DARPin binding domains and linkers</li>
 +
<li>Finalized some biobrick orders</li>
 +
<li>Finalized synthesis order (minus linker)</li>
 +
</ul>
 +
 +
</div>
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
</body>
</body>
</html>
</html>

Revision as of 10:34, 15 September 2012

Penn 2012 iGEM Wiki

June 2012 Notebook

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Week 1

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

Week 2

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Week 3

June 18th

  

Wet Lab

  • Miniprep pDawn and pDusk
  • Test cut pDawn and pDusk with XmaI, analytical gel was correct
  • Prep cut pDawn and pDusk with BamHI and NotI, gel purified
  

Dry Lab

  • Ordered and picked up PCR purification kit from cell center
  • Additional orders through cell center
  • Designed primers for one of Peter's components (forgot which)

June 20

  

Wet Lab

  • Picked 2 colonies of pDawn-mCherry, innoculated in 5 mL of LB and 50 ug/mL of Kan
  • PCR purified fragments (Peter), then ran gel?
  

Dry Lab

  • Researched DARPin binding domains and linkers
  • Finalized some biobrick orders
  • Finalized synthesis order (minus linker)

Retrieved from "http://2012.igem.org/Team:Penn/Notebook"