Team:Penn/Notebook

From 2012.igem.org

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                                                 </ul>                     
                                                 </ul>                     
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+
 +
<p><b>June 11th</b></p>
 +
                            &nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>PCR'd mCherry from NAS157</li>
 +
                                                  <li>Ran 1% Gel and purified product</li>
 +
 
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Designed primers for LsR promoter</li>
 +
<li>Meeting with Dr. Sarkar</li>
 +
</ul>
 +
<br>
 +
<p><b>June 12th</b></p>
 +
                            &nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>Digested mCherry PCR product with BamHI and NotI</li>
 +
                                                  <li>Column purified mCherry and ligated into NAS152 backbone</li>
 +
<li>Transformed NAS152-mCherry into DH5alpha</li>
 +
<li>Poured 25 LB-Kan plates</li>
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Research more information about bacterial drug delivery system</li>
 +
<li>More research into biofilm project</li>
 +
</ul>
 +
<br>
 +
<p><b>June 14th</b></p>
 +
&nbsp;&nbsp;<p>Wet Lab</p>
 +
                                                <ul>
 +
                                                  <li>Fill in later....</li>
 +
                                               
 +
                                                </ul>
 +
&nbsp;&nbsp;<p>Dry Lab</p>
 +
<ul>
 +
<li>Met with Dr. Goulian, obtained pDawn and pDusk</li>
 +
<li>Identified inaK as a surface display gene we can use</li>
 +
</ul>
</div>
</div>

Revision as of 10:28, 15 September 2012

Penn 2012 iGEM Wiki

June 2012

June 6th

  • Set up some lab equipment
  • Autoclaved for a while
  • Organized biobrick stuff
  • Called Vinoo about DNA planning

June 7th

  • Transformed Cph8, pLsr, and LuxS
  • Placed order with Vinoo
  • Developed idea using PGY/PCN system to activate a gene

June 11th

  

Wet Lab

  • PCR'd mCherry from NAS157
  • Ran 1% Gel and purified product
  

Dry Lab

  • Designed primers for LsR promoter
  • Meeting with Dr. Sarkar

June 12th

  

Wet Lab

  • Digested mCherry PCR product with BamHI and NotI
  • Column purified mCherry and ligated into NAS152 backbone
  • Transformed NAS152-mCherry into DH5alpha
  • Poured 25 LB-Kan plates
  

Dry Lab

  • Research more information about bacterial drug delivery system
  • More research into biofilm project

June 14th

  

Wet Lab

  • Fill in later....
  

Dry Lab

  • Met with Dr. Goulian, obtained pDawn and pDusk
  • Identified inaK as a surface display gene we can use

Download

Download the Vallenato script here:

Vallenato

Installation

First, unzip the "vallenato.zip" file and place the "vallenato" folder in the same directory as the html file(s) that will be using the script. If placed in a different folder, you will need to update the paths below.

In the <head> section of your html you will need to link to jQuery, the Vallenato script and stylesheet. You can use the following code:

<script src="http://code.jquery.com/jquery-latest.js" type="text/javascript"></script>

<script src="vallenato/vallenato.js" type="text/javascript"></script>

<link rel="stylesheet" href="vallenato/vallenato.css" type="text/css" media="screen">

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