Team:Macquarie Australia/trial

From 2012.igem.org

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Revision as of 09:23, 15 September 2012

To browse through our notebook simply click on the image with the date. It will expand with what we did that week. To continue on simply click on the next one.

Week 1- Tuesday July 31st

With the break between semesters over the Macquarie iGEM returned to classes. For us this was the first day of iGEM 2012. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced the team and we were began to determine who would take on certain roles within the team.

Gibson Cloning was introduced to all of us for the first time and we eagerly began our project. We had decided to develop a gene-switch controlled by light. To do this a couple of genes would be needed:

a bacteriophytochrome
Heme oxygenase

The bacteriophytochromes from Deinococcus radiodurans and Agrobacterium tumefaciens were chosen. Over the next week Matt Stclair started to develop the G-blocks by:

Acquiring the DNA sequence
Translating into the protein sequence
Using the DNA sequence, optimise codon usage for E. coli
Translating into protein sequence and ensuring no changes in amino acids relative to the original sequence

Week 2- Tuesday 7th August

Our lab work began today with the preparation of liquid media, plates and buffers. The protocols we followed are located here.

We prepared the following:

Liquid LB Media
SOC Solution
SOB Solution
LB Agar Plates
Ampicillin LB Agar Plates: 31 plates
Chloramphenicol LB Agar Plates: 33 Plates
Kanamyacin LB Agar Plates: 32 Plates
TB buffer.
TAE buffer.
EDTA buffer.

Ellaina, Matt Smith, Matt Stclair, Harry, Gazelle, Silas, and Miguel all worked on designing our G Blocks.

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