From 2012.igem.org

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Contents 1 Introduction: Motivation 2 Primary Objective: A Useful BioBrick for Synthetic Biologists 3 Secondary Objective: Humanistic Practice 4 Further Considerations

Introduction: Motivation

• We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.

• We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.

• We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.
  

Abstract/Introduction

Motivation question

Humanistic implications go here

Primary Objective: A Useful BioBrick for Synthetic Biologists

We believe the development of this unprecedented BioBrick will help synthetic biologists in a variety of applications, for a variety of purposes such as the following:

1. example application
2. example application
3. example application

Our proposed BioBrick is novel, and potentially very useful in practice.

Secondary Objective: Humanistic Practice

FAQ/Terminology in engineering Escherichia coli to monitor these variables via fluorescence. Find out more about Carnegie Mellon: (CMU Home Page).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

• Considered aspects of scale-up, including the ethical, legal and social implications of our BioBrick,
• Programmed a new piece of software for modeling our BioBrick to students,
• Developed and tested techniques for measuring translational efficiency and transcriptional strength,
• Participated in human practices demonstration xxx.