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Team:Cambridge/Protocols/GetSpores
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==Method== | ==Method== | ||
| - | *Pick a sing colony from a new plate of B. subtilis and use to inoculate 50ml | + | *Pick a sing colony from a new plate of B. subtilis and use to inoculate 50ml [[Team:Cambridge/Protocols/SporeMedia|<u><span style="color:#00000CD">CDSM</span></u>]]. |
*Grow this for 11 hours in a 37<sup>o</sup>C incubator with vigorous aeration. | *Grow this for 11 hours in a 37<sup>o</sup>C incubator with vigorous aeration. | ||
| - | *Transfer 2-5% of this to make up another 50ml volume of | + | *Transfer 2-5% of this to make up another 50ml volume of [[Team:Cambridge/Protocols/SporeMedia|<u><span style="color:#00000CD">CDSM</span></u>]] culture and grow both for a total incubation time of 48 hours. |
*Longer incubation may be needed for full release of spores. | *Longer incubation may be needed for full release of spores. | ||
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | ||
Revision as of 08:41, 14 September 2012
Producing high yields of B.subtilis spores
Preparation
Plate and grow B. subtilis overnight on antibiotic resistant plates.
Method
- Pick a sing colony from a new plate of B. subtilis and use to inoculate 50ml CDSM.
- Grow this for 11 hours in a 37oC incubator with vigorous aeration.
- Transfer 2-5% of this to make up another 50ml volume of CDSM culture and grow both for a total incubation time of 48 hours.
- Longer incubation may be needed for full release of spores.
