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Team:Macquarie Australia/trial
From 2012.igem.org
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<li>Heme oxygenase</li> | <li>Heme oxygenase</li> | ||
</ol> | </ol> | ||
| - | The bacteriophytochromes from <i>Deinococcus radiodurans</i> and <i>Agrobacterium tumefaciens</i> were chosen. | + | The bacteriophytochromes from <i>Deinococcus radiodurans</i> and <i>Agrobacterium tumefaciens</i> were chosen. Over the next week Matt Stclair started to develop the G-blocks by: |
| + | <ol> | ||
| + | <li>Acquiring the DNA sequence</li> | ||
| + | <li>Translate into protein sequence </li> | ||
| + | <li>Using the DNA sequence, optimise codon usage for <i>E. coli</i></li> | ||
| + | <li>Translate into protein sequence and ensure no changes in amino acids relative to the original sequence </li> | ||
| + | </ol> | ||
| + | </div> | ||
<img src="https://static.igem.org/mediawiki/2012/8/85/Protocol_content_bottom.png" id="bot-part"></img> | <img src="https://static.igem.org/mediawiki/2012/8/85/Protocol_content_bottom.png" id="bot-part"></img> | ||
</div> | </div> | ||
Revision as of 04:45, 14 September 2012
With the break between semester over the Macquarie iGEM returned to classes. For us this was the first day of iGEM 2012. We had our first meeting and discussed our project. Dr. Louise Brown and Associate Professor Rob Willows were introduced the team and we were began to determine who would take on certain roles within the team
Gibson Cloning was introduced to all of us for the first time and we eagerly began our project. We had decided to develop a gene-switch controlled by light. To do this a couple of genes would be needed:
- a bacteriophytochrome
- Heme oxygenase
- Acquiring the DNA sequence
- Translate into protein sequence
- Using the DNA sequence, optimise codon usage for E. coli
- Translate into protein sequence and ensure no changes in amino acids relative to the original sequence
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
This is a trial. I do not know if this is going to work or not!
