Team:TU Darmstadt/Materials/tctB162

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Revision as of 00:27, 14 September 2012

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K808003 tctB162]

Primers for the construction of a BioBrick containing the small subunit B1 of the tripartite tricarboxylate transporter family.

tctB162 BioBrick 

     5' BioBrick                      Annealing 3'
fwd: gtttcttcgaattcgcggccgcttctagatgaaaatcaagagccaaaaagactttttctc
rev: gtttcttcctgcagcggccgctactagtattagccagcgatgaagctaggcc

tctB162 Pst1

Primers for removing a Pst1 site. 

     5'                             3'
fwd: GGCGCTGAATCTCCAGTTCCCCGTGTGGCC
rev: GGCCACACGGGGAACTGGAGATTCAGCGCC
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