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Team:Penn/Notebook/060612
From 2012.igem.org
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[http://en.wikipedia.org/wiki/Wikipedia:Cheatsheet Wikipedia Cheat Sheet] | [http://en.wikipedia.org/wiki/Wikipedia:Cheatsheet Wikipedia Cheat Sheet] | ||
| - | == | + | |
| + | ==6/6/12== | ||
*Set up equipment | *Set up equipment | ||
| - | *Autoclaved 2x 1L Sterile | + | *Autoclaved 2x 1L Sterile ddH<sub>2</sub>O |
| - | *Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp) | + | *Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp) |
| - | * | + | *Added iGEM logo to wiki |
| - | * | + | ==6/7/12== |
| + | *Resuspended available biobricks luxS & plsr (resuspended in 10uL ddH<sub>2</sub>O) | ||
| + | *Resuspended DNA transformed into DH5α (40uL DH5α+2uL DNA) & plated on LB plates w/ 15 uL of 100 mg/mL Amp spread on surface | ||
| + | *Requested lsrR & lsrK from iGEM HQ | ||
| + | ==6/8/12== | ||
| + | *iGEM Wiki now split into two separate Notebooks | ||
| + | *Plenty of colonies, too many to count(TMTC) grew from plsr & luxS transformation from 6/7/12 | ||
| + | **Selected one colony each | ||
| + | **Grew in 10 mL LB+Amp (100 ug/mL) | ||
| + | *Contacted researchers for V. harveyi bioassay & lysostaphin, V. harveyi must be purchased through ATCC ($300), lysostaphin obtained thru MTA. | ||
| + | ==6/9/12== | ||
| + | *~2mL of LB evaporated during incubation | ||
| + | *Cells spun down @ 5000 rpm for 10 min | ||
| + | *Miniprepped 4x Lysis into 1 column | ||
| + | *[plsr]=112 ng/uL | ||
| + | *[luxS]=114 ng/uL | ||
| + | |||
==Mike== | ==Mike== | ||
Revision as of 23:44, 9 June 2012
June 6th, 2012
[http://en.wikipedia.org/wiki/Wikipedia:Cheatsheet Wikipedia Cheat Sheet]
Contents |
6/6/12
- Set up equipment
- Autoclaved 2x 1L Sterile ddH2O
- Autoclaved 500 mL LB Broth for scale up of luxS (Plate 3 Well 2H, pSB1A2), plsr (Plate 2 Well 14H in pSB1A2 resistance to Amp)
- Added iGEM logo to wiki
6/7/12
- Resuspended available biobricks luxS & plsr (resuspended in 10uL ddH2O)
- Resuspended DNA transformed into DH5α (40uL DH5α+2uL DNA) & plated on LB plates w/ 15 uL of 100 mg/mL Amp spread on surface
- Requested lsrR & lsrK from iGEM HQ
6/8/12
- iGEM Wiki now split into two separate Notebooks
- Plenty of colonies, too many to count(TMTC) grew from plsr & luxS transformation from 6/7/12
- Selected one colony each
- Grew in 10 mL LB+Amp (100 ug/mL)
- Contacted researchers for V. harveyi bioassay & lysostaphin, V. harveyi must be purchased through ATCC ($300), lysostaphin obtained thru MTA.
6/9/12
- ~2mL of LB evaporated during incubation
- Cells spun down @ 5000 rpm for 10 min
- Miniprepped 4x Lysis into 1 column
- [plsr]=112 ng/uL
- [luxS]=114 ng/uL
