Team:Bielefeld-Germany/Test

From 2012.igem.org

(Difference between revisions)
(Created page with "{{Team:Bielefeld/Head}} <html> <body> <h1 align=center> Our Project - Abstract</h1> <p align=justify> The birth control pill is a widespread contraception method. However, ...")
Line 1: Line 1:
-
{{Team:Bielefeld/Head}}
+
<html>
 +
<style type="text/css">
 +
 +
ul {list-style-image:none;}
 +
#bodyContent{
 +
    background-color: white;
 +
}
 +
 +
</style>
 +
 +
<div id="tabs-left" class="tabs">
 +
<ul>
 +
<li><a href="#tabs-left-1" name="tabs-left">First</a></li>
 +
<li><a href="#tabs-left-2">Second</a></li>
 +
<li><a href="#tabs-left-3">Third</a></li>
 +
</ul>
 +
 +
<div id="tabs-left-1">
 +
<p>Tabs on the left.</p>
 +
<pre><code class="css">#tabs-left {
 +
position: relative;
 +
padding-left: 6.5em;
 +
}
 +
</div>
 +
<div id="tabs-left-2">
 +
<p>Nunc eleifend iaculis nibh, sed semper nisl feugiat nec. Duis pretium,
 +
felis nec ornare posuere, leo erat ullamcorper nisl, a lacinia dolor urna vel quam!</p>
 +
</div>
 +
<div id="tabs-left-3">
 +
<p>Pellentesque ac elit et nulla posuere convallis quis ut dolor. Pellentesque egestas
 +
pellentesque blandit. Morbi quis sapien nec lacus consectetur vestibulum.</p>
 +
</div>
 +
</div>
 +
 +
 +
 +
<!-- navigator -->
 +
<div id="nav">
 +
<ul style="list-style-type:none">
 +
<li>
 +
<a href="#1">
 +
<img src="https://static.igem.org/mediawiki/2011/9/9a/Bielefeld-Germany2011-Thermocycler-klein.jpg"/>
 +
<strong>Molecular</strong>
 +
Genetic engineering protocols
 +
</a>
 +
</li>
 +
<li>
 +
<a href="#2">
 +
<img src="https://static.igem.org/mediawiki/2012/b/bc/Bielefeld2012_Production.jpg"/>
 +
<strong>Production</strong>
 +
Upstreaming and downstreaming
 +
</a>
 +
 +
</li>
 +
<li>
 +
<a href="#3">
 +
<img src="https://static.igem.org/mediawiki/2012/a/a2/Bielefeld2012_Activity_75.jpg" />
 +
<strong>Analytics</strong>
 +
Analytical methods
 +
</a>
 +
</li>
 +
<li>
 +
<a href="#4">
 +
<img src="https://static.igem.org/mediawiki/2011/9/9a/Bielefeld-Germany2011-Thermocycler-klein.jpg"/>
 +
<strong>Immobilization</strong>
 +
 +
</a>
 +
</li>
 +
<li>
 +
<a href="#5">
 +
<img src="https://static.igem.org/mediawiki/2011/6/6c/Bielefeld-Germany2011-analyticsklein.JPG" />
 +
<strong>Material</strong>
 +
Enzymes, kits, chemicals, buffers
 +
</a>
 +
</li>
 +
</ul>
 +
</div>
 +
 +
<!-- tab panes -->
 +
<div id="panes">
 +
 +
<div>
 +
<img src="https://static.igem.org/mediawiki/2011/thumb/1/1a/Bielefeld_Silver_1.png/300px-Bielefeld_Silver_1.png"  />
 +
 +
<h3 style="text-decoration:none; color:black;">Molecular</h3>
 +
 +
<p class="more">
 +
In this section of our protocol pages you can read more about our methods for cloning and BioBrick assembly.
 +
</p>
 +
 +
<p>
 +
Genetic engineering is a basic tool of synthetic biology. With the help of standardized DNA building blocks (BioBricks) it is fairly easy to create new and modify existing natural systems. The methods we have used in our project to create BioBricks and to modify, mutate, transform and analyse DNA are presented in this section. Methods used: Electroporation; chemical transformation; Standard, Freiburg, Gibson and 3A BioBrick assembly; restriction analysis; colony PCR; site directed mutagenesis. <a href="https://2012.igem.org/Team:Bielefeld-Germany/Protocols/molecular_genetics">read more</a>
 +
</p>
 +
</div>
 +
 +
<div>
 +
 +
<img src="https://static.igem.org/mediawiki/2012/c/c1/Bielefeld2012_Production_300.jpg"  />
 +
 +
<h3 style="text-decoration:none; color:black;">Production</h3>
 +
 +
<p class="more">
 +
These are the protocols for the cultivations and the downstream processing.
 +
</p>
 +
 +
<p>
 +
Before one is able to work with a cell-free system based on biological material, the needed proteins have to be produced and purified first. These methods and the ones we used to characterize BioBricks in vivo are presented in this section. Used methods: Cultivations in shaking flasks and bioreactor; protein clean-up from medium, periplasm, whole cell and inclusion bodies; UF / DF; IEX; Ni-NTA columns and chromatography; recrystallization and immobilization of S-layer proteins. <a href="https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Production">read more</a>
 +
</p>
 +
 +
</div>
 +
 +
<div>
 +
 +
<img src="https://static.igem.org/mediawiki/2012/6/60/Bielefeld2012_Activity_300.jpg"  />
 +
 +
<h3 style="text-decoration:none; color:black;">Analytics</h3>
 +
 +
<p class="more">
 +
Protocols for the analytical methods we used.
 +
</p>
 +
 +
<p>
 +
DNA and proteins are very small and cannot be seen by the naked eye. To control the success and the results of your upstream and downstream processes, analytical methods are necessary that give reliable results to make DNA or proteins in any way visible for you. The analytical methods we used in our project can be found in this section. Used methods: Fluorescence measurement; SDS-PAGE; MALDI-TOF; HPLC; LC-ESI-qTOF-MS/MS; molecular beacons; extraction. <a href="https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Analytics">read more</a>
 +
</p>
 +
 +
</div>
 +
 +
<div>
 +
 +
<img src="https://static.igem.org/mediawiki/2011/2/26/Bielefeld-Germany2011-MaterialMethods300px.JPG"  />
 +
 +
<h3 style="text-decoration:none; color:black;">Immobilization</h3>
 +
 +
<p class="more">
 +
Here you can find out, how we immobilized the produced enzymes.
 +
</p>
 +
 +
<p>
 +
Coming soon <a href="https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Immobilization">read more</a>
 +
</p>
 +
 +
</div>
 +
        <div>
 +
 +
<img src="https://static.igem.org/mediawiki/2011/2/26/Bielefeld-Germany2011-MaterialMethods300px.JPG"  />
 +
 +
<h3 style="text-decoration:none; color:black;" >Material</h3>
 +
 +
<p class="more">
 +
Chemicals, enzymes and kits we used in our lab work.
 +
</p>
 +
 +
<p>
 +
Chemical and biological reactions need defined conditions to work as expected. The chemicals, enzymes, kits, buffers and media we used in our project are listed in this section. <a href="https://2012.igem.org/Team:Bielefeld-Germany/Protocols/Materials">read more</a>
 +
</p>
 +
 +
</div>
-
<html>
 
-
<body>
+
</div>
-
<h1 align=center> Our Project - Abstract</h1>
 
-
<p align=justify>
 
-
The birth control pill is a widespread contraception method. However, large amounts of these modified estrogens leave the body again in urine. The conventional methods in sewage treatment plants are unable to treat waste water sufficiently because the most frequently used estrogen ethinylestradiol is very difficult to break down. As a result, the hormone finds its way into rivers and lakes and accumulates in drinking water with serious consequences for fish and other aquatic life. These range from reproductive and severe developmental disorders to the formation of female sexual characteristics in males. The long-term consequences of increasing estrogen pollution for human beings are still largely unknown. Nonetheless, declining sperm counts and thereby increasing infertility in men living in industrial nations may well relate to this hormonal pollution. In addition, testicular and prostate cancers as well as osteoporosis could be a consequence of overly high concentrations of estrogen in the human body.<br><br>
 
-
The goal of Bielefeld’s iGEM team is to develop a biological filter using immobilized laccases to purify municipal and industrial wastewater from synthetic estrogens and other aromatic compounds. Laccases are copper-containing oxidase enzymes found in many organisms, and one of their properties is the ability to break down a wide range of aromatic and phenolic compounds. For this purpose, genes of various bacterial and eukaryotic laccases should be isolated and expressed in Escherichia coli and Pichia pastoris. The team is aiming to manufacture this enzyme economically and safely with the help of methods from synthetic biology. It should also be possible to extend the concept to other, in part poisonous and carcinogenic pollutants in drinking and waste water, as well as into industrial application, such as in paper and textile industries or even for bioremediation of contaminated soil. </p>
 
 +
<script>
 +
$(function() {
 +
 +
 +
$("#nav ul").tabs("#panes > div", {effect: 'fade', fadeOutSpeed: 400});
 +
});
 +
</script>
-
<h1 align="center">Introducing the iGEM Team Bielefeld 2012</h1>
 
-
<div align="center" style="z-index:0;"><object width="450" height="280"><param name="movie" value="http://www.youtube.com/v/HwvumBLdwP8?fs=1&amp;hl=en_GB"></param><param name="allowFullScreen" value="true"></param><param name="allowscriptaccess" value="always"></param><param name="wmode" value="opaque" /><embed src="http://www.youtube.com/v/HwvumBLdwP8?fs=1&amp;hl=en_GB" type="application/x-shockwave-flash" allowscriptaccess="always" allowfullscreen="true" width="450" height="280" wmode="opaque"></embed></object></div>
 
-
</body>
 
</html>
</html>

Revision as of 10:56, 13 September 2012

Tabs on the left.

#tabs-left {
	position: relative;
	padding-left: 6.5em;
}

Nunc eleifend iaculis nibh, sed semper nisl feugiat nec. Duis pretium, felis nec ornare posuere, leo erat ullamcorper nisl, a lacinia dolor urna vel quam!

Pellentesque ac elit et nulla posuere convallis quis ut dolor. Pellentesque egestas pellentesque blandit. Morbi quis sapien nec lacus consectetur vestibulum.

Molecular

In this section of our protocol pages you can read more about our methods for cloning and BioBrick assembly.

Genetic engineering is a basic tool of synthetic biology. With the help of standardized DNA building blocks (BioBricks) it is fairly easy to create new and modify existing natural systems. The methods we have used in our project to create BioBricks and to modify, mutate, transform and analyse DNA are presented in this section. Methods used: Electroporation; chemical transformation; Standard, Freiburg, Gibson and 3A BioBrick assembly; restriction analysis; colony PCR; site directed mutagenesis. read more

Production

These are the protocols for the cultivations and the downstream processing.

Before one is able to work with a cell-free system based on biological material, the needed proteins have to be produced and purified first. These methods and the ones we used to characterize BioBricks in vivo are presented in this section. Used methods: Cultivations in shaking flasks and bioreactor; protein clean-up from medium, periplasm, whole cell and inclusion bodies; UF / DF; IEX; Ni-NTA columns and chromatography; recrystallization and immobilization of S-layer proteins. read more

Analytics

Protocols for the analytical methods we used.

DNA and proteins are very small and cannot be seen by the naked eye. To control the success and the results of your upstream and downstream processes, analytical methods are necessary that give reliable results to make DNA or proteins in any way visible for you. The analytical methods we used in our project can be found in this section. Used methods: Fluorescence measurement; SDS-PAGE; MALDI-TOF; HPLC; LC-ESI-qTOF-MS/MS; molecular beacons; extraction. read more

Immobilization

Here you can find out, how we immobilized the produced enzymes.

Coming soon read more

Material

Chemicals, enzymes and kits we used in our lab work.

Chemical and biological reactions need defined conditions to work as expected. The chemicals, enzymes, kits, buffers and media we used in our project are listed in this section. read more

Retrieved from "http://2012.igem.org/Team:Bielefeld-Germany/Test"