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Protocol: PCR
1X Mastermix 20μl reaction
| Reagent | Volume [μl] |
|---|---|
| Water | 20-x |
| HF-Buffer (5x) | 4 |
| DMSO (optional) | 0.6 |
| dNTPs | 0.4 |
| Forward primer (50μM) | 0.2 |
| Reverse primer (50μM) | 0.2 |
| Template (10ng/μl) | 0.5 |
| Phusion HF polymerase | 0.2 |
Team:EPF-Lausanne/Protocol/PCR
From 2012.igem.org
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Revision as of 09:58, 13 September 2012
